ArticleBioMed research international2014
Effect of inflammatory mediators on ATP release of human urothelial RT4 cells.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Mechanosensitive release of ATP in the urinary bladder mucosa.Purinergic signalling · 2025Review
- Prostaglandins Differentially Regulate the Constitutive and Mechanosensitive Release of Soluble Nucleotidases in the Urinary Bladder Mucosa.International journal of molecular sciences · 2024Article
- Effect of pro-inflammatory cytokines on urothelial cell adenosine triphosphate release and breakdown.Bladder (San Francisco, Calif.) · 2024Article
- The mast cell stimulator compound 48/80 causes urothelium-dependent increases in murine urinary bladder contractility.American journal of physiology. Renal physiology · 2023Article
- Purinergic signalling in the urinary bladder - When function becomes dysfunction.Autonomic neuroscience : basic & clinical · 2021Article
- P2X7 Receptor Blockade Protects Against Acrolein-Induced Bladder Damage: A Potential New Therapeutic Approach for the Treatment of Bladder Inflammatory Diseases.Frontiers in pharmacology · 2021Article
- Purinergic receptor mediated calcium signalling in urothelial cells.Scientific reports · 2019Article
- Feline Interstitial Cystitis Enhances Mucosa-Dependent Contractile Responses to Serotonin.International neurourology journal · 2018Article
- Modulation of lower urinary tract smooth muscle contraction and relaxation by the urothelium.Naunyn-Schmiedeberg's archives of pharmacology · 2018Review
- Effect of short-term androgen deficiency on bladder contractility and urothelial mediator release.Naunyn-Schmiedeberg's archives of pharmacology · 2017Article
- Article
- Recovery of urothelial mediator release but prolonged elevations in interleukin-8 and nitric oxide secretion following mitomycin C treatment.Naunyn-Schmiedeberg's archives of pharmacology · 2015Article
- Local receptors as novel regulators for peripheral clock expression.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inflammation is an important contributor to the aetiology of a number of bladder dysfunctions including interstitial cystitis, painful bladder syndrome, and overactive bladder. The aim of this study was to examine the effects of inflammatory mediators on urothelial ATP release. Human urothelial RT4 cells were exposed to normal buffer or varying concentrations of inflammatory mediators (bradykinin, histamine, and serotonin) in the presence or absence of hypotonic stretch stimuli (1 : 2 dilution of Krebs-Henseleit buffer). Others have demonstrated that bradykinin increased stretch-induced ATP release; however, we observed no change in control or stretch-induced ATP release with bradykinin. Pretreatment of RT4 cells with histamine or serotonin decreased stretch-induced ATP release (P = 0.037, P = 0.040, resp.). Previous studies have demonstrated increased ATP release in response to inflammation utilising whole bladder preparations in contrast to our simple model of cultured urothelial cells. The current study suggests that it is unlikely that there is a direct interaction between the release of inflammatory mediators and increased ATP release, but rather more complex interactions occurring in response to inflammation that lead to increased bladder sensation.
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