Evidence map›Paper›PMID 24839392›Full record

ArticleCell communication insights2013

Identification of Signaling Protein Complexes by Parallel Affinity Precipitation Coupled with Mass Spectrometry.

Heng Lu, Qishan Lin, Jihe Zhao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell communication insights, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Heng LuBurnett School of Biomedical Science, College of Medicine, University of Central Florida, Orlando, FL, USA.
Qishan LinUniversity at Albany Proteomics Facility, Center for Functional Genomics, Rensselaer, NY, USA.
Jihe ZhaoBurnett School of Biomedical Science, College of Medicine, University of Central Florida, Orlando, FL, USA.

Funding

KLF8 Regulation of EMT Tumor MetastasisR01CA132977 · NCI · UNIVERSITY OF CENTRAL FLORIDA · PI ZHAO, JIHE · 2009 to 2014
$1.3M
Q-TOF MASS SPEC FOR INTERDISCIPLINARY PROTEOMIC RESEARCH: GENETICSS10RR017812 · NCRR · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI CUNNINGHAM, RICHARD P. · 2004 to 2004
$464k
NCI NIH HHS R01 CA132977NCRR NIH HHS S10 RR017812
6 · The paper itself

Abstract

Protein-protein interactions play a pivotal role in both inter- and intra-cellular signaling. Identification of signaling protein complexes can thus shed important new insights into cell communications. We developed a parallel affinity precipitation protocol to overcome the disadvantages of the tandem affinity purification procedure, such as the potential disruption of target protein conformation, subcellular localization or function by epitope tags, the potential need of large amounts of cell culture or generation of stable cell lines, and relatively long duration the two-step precipitation takes. This new simplified assay of protein interaction is quick, economic and specific. This paper describes the details in the design and method of the assay.

Indexed as

co-immunoprecipitation (co-IP)hemagglutinin tagmass spectrometryMyc tagparallel affinity precipitation (PAP)protein–protein interactiontandem affinity purification (TAP)

Identifiers

PMID24839392
PMCPMC4020431

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.