ArticleCell biochemistry and biophysics2014
Laurdan monitors different lipids content in eukaryotic membrane during embryonic neural development.
Article in Cell biochemistry and biophysics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Membrane manipulation by free fatty acids improves microbial plant polyphenol synthesis.Nature communications · 2023Article
- Effect of osmotic stress on live cell plasma membranes, probed via Laurdan general polarization measurements.Biophysical journal · 2022Article
- LAURDAN since Weber: The Quest for Visualizing Membrane Heterogeneity.Accounts of chemical research · 2021Article
- Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids.Current topics in membranes · 2021Review
- Defining Lineage-Specific Membrane Fluidity Signatures that Regulate Adhesion Kinetics.Stem cell reports · 2018Article
- LPS-Induced Macrophage Activation and Plasma Membrane Fluidity Changes are Inhibited Under Oxidative Stress.The Journal of membrane biology · 2016Article
- Assessment of Membrane Fluidity Fluctuations during Cellular Development Reveals Time and Cell Type Specificity.PloS one · 2016Article
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6 authors.
Funding
Abstract
We describe a method based on fluorescence-lifetime imaging microscopy (FLIM) to assess the fluidity of various membranes in neuronal cells at different stages of development [day 12 (E12) and day 16 (E16) of gestation]. For the FLIM measurements, we use the Laurdan probe which is commonly used to assess membrane water penetration in model and in biological membranes using spectral information. Using the FLIM approach, we build a fluidity scale based on calibration with model systems of different lipid compositions. In neuronal cells, we found a marked difference in fluidity between the internal membranes and the plasma membrane, being the plasma membrane the less fluid. However, we found no significant differences between the two cell groups, E12 and E16. Comparison with NIH3T3 cells shows that the plasma membranes of E12 and E16 cells are significantly more fluid than the plasma membrane of the cancer cells.
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