Evidence map›Paper›PMID 24838967›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2014

In vivo electroporation of adult mouse sensory neurons for studying peripheral axon regeneration.

Saijilafu, Bo-Yin Zhang, Feng-Quan Zhou

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 76% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Experimental Model Systems for Understanding Human Axonal Injury Responses.International journal of molecular sciences · 2021
    Review
  2. Telomerase Reverse Transcriptase and p53 Regulate Mammalian Peripheral Nervous System and CNS Axon Regeneration Downstream of c-Myc.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

SaijilafuDepartment of Orthopaedic Surgery, The Johns Hopkins University, Baltimore, MD, USA.
Bo-Yin Zhang
Feng-Quan Zhou
Johns Hopkins Medicine · US

Funding

Epigenetic regulation of neuronal morphogenesis in development and regenerationR01NS085176 · NINDS · JOHNS HOPKINS UNIVERSITY · PI ZHOU, FENGQUAN · 2014 to 2018
$1.8M
Role of CLASP in neuronal morphogenesis during developmentR01NS064288 · NINDS · JOHNS HOPKINS UNIVERSITY · PI ZHOU, FENGQUAN · 2009 to 2013
$1.8M
Epigenetics-mediated transcription regulation in mammalsR01GM111514 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI QIAN, JIANG, ZHOU, FENGQUAN · 2014 to 2017
$1.4M
NIGMS NIH HHS R01 GM111514NINDS NIH HHS R01 NS064288NINDS NIH HHS R01NS064288NINDS NIH HHS R01 NS085176
6 · The paper itself

Abstract

Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain- and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.

Indexed as

Nerve RegenerationAnimalsAxonsDNAElectroporationFemaleGanglia, SpinalMiceMicroinjectionsMicroscopy, FluorescenceNeurosurgical ProceduresOligoribonucleotides, AntisensePlasmidsSciatic NerveSensory Receptor CellsTransfectionDNAOligoribonucleotides, Antisense

Identifiers

PMID24838967
PMCPMC4668268
OpenAlexW2408512355

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.