ArticleMicrobiologyOpen2014
Comparison of DNA extraction kits and modification of DNA elution procedure for the quantitation of subdominant bacteria from piggery effluents with real-time PCR.
Article in MicrobiologyOpen, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Optimization of DNA extraction methods from pig farm wastewater for pathogen detection using metagenomic sequencing.Microbial genomics · 2025Article
- Tracking Different States of Spiked Environmental DNA Using Multiplex Digital PCR Assays.Environmental microbiology · 2025Article
- Optimization of a Protocol for Isolating Cell-free DNA From Cerebrospinal Fluid.Annals of laboratory medicine · 2024Article
- Comparison of DNA extraction methods on different sample matrices within the same terrestrial ecosystem.Scientific reports · 2024Article
- Moving towards on-site detection of Shiga toxin-producingCurrent research in food science · 2024Article
- Estimating the bias related to DNA recovery from hemp stems for retting microbial community investigation.Applied microbiology and biotechnology · 2023Article
- Evaluation of DNA Extraction Methods for Reliable Quantification ofBiosensors · 2023Article
- The Effects of DNA Extraction Kits and Primers on Prokaryotic and Eukaryotic Microbial Community in Freshwater Sediments.Microorganisms · 2022Article
- Diversity of Antibiotic Resistance genes and Transfer Elements-Quantitative Monitoring (DARTE-QM): a method for detection of antimicrobial resistance in environmental samples.Communications biology · 2022Article
- Toward Best Practice in Livestock Microbiota Research: A Comprehensive Comparison of Sample Storage and DNA Extraction Strategies.Frontiers in microbiology · 2021Article
- The Utilization of Linear Polylysine Coupled with Mechanic Forces to Extract Microbial DNA from Different Matrices.Microorganisms · 2020Article
- Optimization of a Method for the Concentration of Genetic Material in Bacterial and Fungal Communities on Fresh Apple Peel Surfaces.Microorganisms · 2020Article
- Comprehensive evaluation of a cost-effective method of culturingBiotechnology for biofuels · 2019Article
- Detection of Clostridium botulinum group III in environmental samples from farms by real-time PCR using four commercial DNA extraction kits.BMC research notes · 2018Article
- Development of a species-specific TaqMan-MGB real-time PCR assay to quantify Olsenella scatoligenes in pigs offered a chicory root-based diet.AMB Express · 2018Article
- The Madness of Microbiome: Attempting To Find Consensus "Best Practice" for 16S Microbiome Studies.Applied and environmental microbiology · 2018Review
- Improving detection of avian malaria from host blood: a step towards a standardised protocol for diagnostics.Parasitology research · 2016Article
- Evaluation of DNA extraction kits and phylogenetic diversity of the porcine gastrointestinal tract based on Illumina sequencing of two hypervariable regions.MicrobiologyOpen · 2016Article
- Fate of Viable but Non-culturable Listeria monocytogenes in Pig Manure Microcosms.Frontiers in microbiology · 2016Article
- Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents.BMC microbiology · 2015Article
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2 authors.
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Abstract
Four commercial DNA extraction kits and a minor modification in the DNA elution procedure were evaluated for the quantitation of bacteria in pig manure samples. The PowerSoil(®), PowerFecal(®), NucleoSpin(®) Soil kits and QIAamp(®) DNA Stool Mini kit were tested on raw manure samples and on lagoon effluents for their ability to quantify total bacteria and a subdominant bacteria specific of pig manure contamination: Lactobacillus amylovorus. The NucleoSpin(®) Soil kit (NS kit), and to a lesser extent the PowerFecal(®) kit were the most efficient methods. Regardless of the kit utilized, the modified elution procedure increased DNA yield in the lagoon effluent by a factor of 1.4 to 1.8. When tested on 10 piggery effluent samples, compared to the QIAamp kit, the NS kit combined with the modified elution step, increased by a factor up to 1.7 log10 the values of the concentration of L. amylovorus. Regardless of the type of manure, the best DNA quality and the highest concentrations of bacteria were obtained using the NS kit combined with the modification of the elution procedure. The method recommended here significantly improved quantitation of subdominant bacteria in manure.
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