ArticleNeuroscience bulletin2014
Generation of a monkey with MECP2 mutations by TALEN-based gene targeting.
Article in Neuroscience bulletin, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 53 citations in OpenAlex.
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- A Blueprint for Translational Precision Medicine in Autism Spectrum Disorder and Related Neurogenetic Syndromes.Journal of child and adolescent psychopharmacology · 2025Review
- The Role of Nonhuman Primates in Neurotoxicology Research: Preclinical Models and Experimental Methods.Current protocols · 2023Article
- Review
- Modeling genetic diseases in nonhuman primates through embryonic and germline modification: Considerations and challenges.Science translational medicine · 2022Review
- Gene editing monkeys: Retrospect and outlook.Frontiers in cell and developmental biology · 2022Review
- The dawn of non-human primate models for neurodevelopmental disorders.Current opinion in genetics & development · 2020Review
- Efficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing.Scientific reports · 2019Article
- Local transgene expression and whole-body transgenesis to model brain diseases in nonhuman primate.Animal models and experimental medicine · 2019Review
- Generation of genetically engineered non-human primate models of brain function and neurological disorders.American journal of primatology · 2019Review
- Homologous recombination-mediated targeted integration in monkey embryos using TALE nucleases.BMC biotechnology · 2019Article
- Cloning of a gene-edited macaque monkey by somatic cell nuclear transfer.National science review · 2019Article
- Modeling autism in non-human primates: Opportunities and challenges.Autism research : official journal of the International Society for Autism Research · 2018Review
- Responsible innovation in human germline gene editing: Background document to the recommendations of ESHG and ESHRE.European journal of human genetics : EJHG · 2018Article
- Genetic engineering in nonhuman primates for human disease modeling.Journal of human genetics · 2018Review
- Rett syndrome from bench to bedside: recent advances.F1000Research · 2018Review
- Responsible innovation in human germline gene editing. Background document to the recommendations of ESHG and ESHRE.Human reproduction open · 2018Article
- Non-human Primate Models for Brain Disorders - Towards Genetic Manipulations via Innovative Technology.Neuroscience bulletin · 2017Review
- Germ line genome editing in clinics: the approaches, objectives and global society.Briefings in functional genomics · 2017Review
- Genome-Editing Technologies: Principles and Applications.Cold Spring Harbor perspectives in biology · 2016Review
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene editing in model organisms has provided critical insights into brain development and diseases. Here, we report the generation of a cynomolgus monkey (Macaca fascicularis) carrying MECP2 mutations using transcription activator-like effector nucleases (TALENs)-mediated gene targeting. After injecting TALENs mRNA into monkey zygotes achieved by in vitro fertilization and embryo transplantation into surrogate monkeys, we obtained one male newborn monkey with an MECP2 deletion caused by frameshifting mutation in various tissues. The monkey carrying the MECP2 mutation failed to survive after birth, due to either the toxicity of TALENs or the critical requirement of MECP2 for neural development. The level of MeCP2 protein was essentially depleted in the monkey's brain. This study demonstrates the feasibility of introducing genetic mutations in non-human primates by site-specific gene-editing methods.
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