ArticleApplied and environmental microbiology2014
Novel microarray design for molecular serotyping of shiga toxin- producing Escherichia coli strains isolated from fresh produce.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli.Frontiers in cellular and infection microbiology · 2015Pooled it
- Molecular characterization ofVeterinary world · 2021Article
- A Targeted Sequencing Assay for SerotypingFrontiers in microbiology · 2020Article
- Multiplex PCR Assays for the Detection of One Hundred and Thirty Seven Serogroups of Shiga Toxin-ProducingFrontiers in cellular and infection microbiology · 2020Article
- Article
- Interlaboratory Evaluation of the U.S. Food and Drug Administration Escherichia coli Identification Microarray for Profiling Shiga Toxin-Producing Escherichia coli.Journal of food protection · 2018Article
- High-Quality Complete Genome Sequences of Three Bovine Shiga Toxin-ProducingGenome announcements · 2018Article
- Article
- Establishment of a Molecular Serotyping Scheme and a Multiplexed Luminex-Based Array forFrontiers in microbiology · 2018Article
- FDA Escherichia coli Identification (FDA-ECID) Microarray: a Pangenome Molecular Toolbox for Serotyping, Virulence Profiling, Molecular Epidemiology, and Phylogeny.Applied and environmental microbiology · 2016Article
- Advances in Molecular Serotyping and Subtyping of Escherichia coli.Frontiers in microbiology · 2016Review
- Characterization of Shiga Toxin Subtypes and Virulence Genes in Porcine Shiga Toxin-Producing Escherichia coli.Frontiers in microbiology · 2016Article
- Article
- Virulence Potential of Activatable Shiga Toxin 2d-Producing Escherichia coli Isolates from Fresh Produce.Journal of food protection · 2015Article
- A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster.DNA research : an international journal for rapid publication of reports on genes and genomes · 2015Article
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5 authors.
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Abstract
Serotyping Escherichia coli is a cumbersome and complex procedure due to the existence of large numbers of O- and H-antigen types. It can also be unreliable, as many Shiga toxin-producing E. coli (STEC) strains isolated from fresh produce cannot be typed by serology or have only partial serotypes. The FDA E. coli identification (FDA-ECID) microarray, designed for characterizing pathogenic E. coli, contains a molecular serotyping component, which was evaluated here for its efficacy. Analysis of a panel of 75 reference E. coli strains showed that the array correctly identified the O and H types in 97% and 98% of the strains, respectively. Comparative analysis of 73 produce STEC strains showed that serology and the array identified 37% and 50% of the O types, respectively, and that the array was able to identify 16 strains that could not be O serotyped. Furthermore, the array identified the H types of 97% of the produce STEC strains compared to 65% by serology, including six strains that were mistyped by serology. These results show that the array is an effective alternative to serology in serotyping environmental E. coli isolates.
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