ArticleApplied and environmental microbiology2014
Sensitive detection of Xanthomonas oryzae Pathovars oryzae and oryzicola by loop-mediated isothermal amplification.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 49 citations in OpenAlex.
- Electrochemical DNA biosensor based on a novel pyrrolidinyl peptide nucleic acid sequence immobilized on a graphene oxide/bovine serum albumin-modified electrode for the direct diagnosis of seed-borne pathogen.Mikrochimica acta · 2025Article
- Genome guided LAMP assay for rapid and reliable detection of Xanthomonas axonopodis pv. vasculorum.Scientific reports · 2025Article
- Evaluating Xanthomonas oryzae pv. oryzae (Xoo) infection dynamics in rice for distribution routes and environmental reservoirs by molecular approaches.Scientific reports · 2025Article
- Taxonogenomic analysis of theInternational journal of systematic and evolutionary microbiology · 2024Article
- Trends in Molecular Diagnosis and Diversity Studies for Phytosanitary RegulatedMicroorganisms · 2021Review
- Development of Real-Time and Colorimetric Loop Mediated Isothermal Amplification Assay for Detection ofMicroorganisms · 2020Article
- Rice Galaxy: an open resource for plant science.GigaScience · 2019Article
- A Pathovar ofFrontiers in plant science · 2019Article
- Development of a genome-informed loop-mediated isothermal amplification assay for rapid and specific detection of Xanthomonas euvesicatoria.Scientific reports · 2018Article
- Pest categorisation ofEFSA journal. European Food Safety Authority · 2018Article
- Population genomic insights into variation and evolution of Xanthomonas oryzae pv. oryzae.Scientific reports · 2017Article
- LAMP Detection Assays for Boxwood Blight Pathogens: A Comparative Genomics Approach.Scientific reports · 2016Article
- Article
- Genomic-associated Markers and comparative Genome Maps of Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola.World journal of microbiology & biotechnology · 2015Article
- New multilocus variable-number tandem-repeat analysis tool for surveillance and local epidemiology of bacterial leaf blight and bacterial leaf streak of rice caused by Xanthomonas oryzae.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular diagnostics for crop diseases can enhance food security by enabling the rapid identification of threatening pathogens and providing critical information for the deployment of disease management strategies. Loop-mediated isothermal amplification (LAMP) is a PCR-based tool that allows the rapid, highly specific amplification of target DNA sequences at a single temperature and is thus ideal for field-level diagnosis of plant diseases. We developed primers highly specific for two globally important rice pathogens, Xanthomonas oryzae pv. oryzae, the causal agent of bacterial blight (BB) disease, and X. oryzae pv. oryzicola, the causal agent of bacterial leaf streak disease (BLS), for use in reliable, sensitive LAMP assays. In addition to pathovar distinction, two assays that differentiate X. oryzae pv. oryzae by African or Asian lineage were developed. Using these LAMP primer sets, the presence of each pathogen was detected from DNA and bacterial cells, as well as leaf and seed samples. Thresholds of detection for all assays were consistently 10(4) to 10(5) CFU ml(-1), while genomic DNA thresholds were between 1 pg and 10 fg. Use of the unique sequences combined with the LAMP assay provides a sensitive, accurate, rapid, simple, and inexpensive protocol to detect both BB and BLS pathogens.
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