ArticleApplied and environmental microbiology2014
Characterization of a nitrilase and a nitrile hydratase from Pseudomonas sp. strain UW4 that converts indole-3-acetonitrile to indole-3-acetic acid.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 56 citations in OpenAlex.
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- Biosynthetic Pathways and Functions of Indole-3-Acetic Acid in Microorganisms.Microorganisms · 2023Review
- Phytoremediation as an Effective Remedy for Removing Trace Elements from Ecosystems.Plants (Basel, Switzerland) · 2023Review
- Screening and characterization of a nitrilase with significant nitrile hydratase activity.Biotechnology letters · 2022Article
- Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa.Biotechnology for biofuels and bioproducts · 2022Article
- Plant growth-promoting effect and genomic analysis of the P. putida LWPZF isolated from C. japonicum rhizosphere.AMB Express · 2022Article
- Draft Genome Sequence of Pseudomonas sp. Strain RGM 3321, a Phyllosphere Endophyte from Fragaria chiloensis subsp.Microbiology resource announcements · 2022Article
- Rational Regulation of Reaction Specificity of Nitrilase for Efficient Biosynthesis of 2-Chloronicotinic Acid through a Single Site Mutation.Applied and environmental microbiology · 2022Article
- Metabolism of Aldoximes and Nitriles in Plant-Associated Bacteria and Its Potential in Plant-Bacteria Interactions.Microorganisms · 2022Review
- Identification and Characterization of Auxin/IAA Biosynthesis Pathway in the Rice Blast FungusJournal of fungi (Basel, Switzerland) · 2022Article
- From sequence to function: a new workflow for nitrilase identification.Applied microbiology and biotechnology · 2020Article
- Advances in cloning, structural and bioremediation aspects of nitrile hydratases.Molecular biology reports · 2019Review
- Cloning and functional characterization of nitrilase from Fusarium proliferatum AUF-2 for detoxification of nitriles.Functional & integrative genomics · 2015Article
- Microbial metabolism marvels: a comprehensive review of microbial drug transformation capabilities.Gut microbesReview
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Indole-3-acetic acid (IAA) is a fundamental phytohormone with the ability to control many aspects of plant growth and development. Pseudomonas sp. strain UW4 is a rhizospheric plant growth-promoting bacterium that produces and secretes IAA. While several putative IAA biosynthetic genes have been reported in this bacterium, the pathways leading to the production of IAA in strain UW4 are unclear. Here, the presence of the indole-3-acetamide (IAM) and indole-3-acetaldoxime/indole-3-acetonitrile (IAOx/IAN) pathways of IAA biosynthesis is described, and the specific role of two of the enzymes (nitrilase and nitrile hydratase) that mediate these pathways is assessed. The genes encoding these two enzymes were expressed in Escherichia coli, and the enzymes were isolated and characterized. Substrate-feeding assays indicate that the nitrilase produces both IAM and IAA from the IAN substrate, while the nitrile hydratase only produces IAM. The two nitrile-hydrolyzing enzymes have very different temperature and pH optimums. Nitrilase prefers a temperature of 50°C and a pH of 6, while nitrile hydratase prefers 4°C and a pH of 7.5. Based on multiple sequence alignments and motif analyses, physicochemical properties and enzyme assays, it is concluded that the UW4 nitrilase has an aromatic substrate specificity. The nitrile hydratase is identified as an iron-type metalloenzyme that does not require the help of a P47K activator protein to be active. These data are interpreted in terms of a preliminary model for the biosynthesis of IAA in this bacterium.
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