ArticleJournal of bacteriology2014
Control of natural transformation in salivarius Streptococci through specific degradation of σX by the MecA-ClpCP protease complex.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Fluoride triggers lysis inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- MecA: A Multifunctional ClpP-Dependent and Independent Regulator in Gram-Positive Bacteria.Molecular microbiology · 2025Review
- MecA inmSphere · 2024Article
- Unveiling the regulatory network controlling natural transformation in lactococci.PLoS genetics · 2024Article
- Mass spectrometry and split luciferase complementation assays reveal the MecA protein interactome ofMicrobiology spectrum · 2024Article
- Article
- Expanding natural transformation to improve beneficial lactic acid bacteria.FEMS microbiology reviews · 2022Article
- Clp ATPases differentially affect natural competence development in Streptococcus mutans.MicrobiologyOpen · 2022Article
- ClpX/P-Dependent Degradation of Novel Substrates in Streptococcus mutans.Journal of bacteriology · 2022Article
- Protein degradation control and regulation of bacterial survival and pathogenicity: the role of protein degradation systems in bacteria.Molecular biology reports · 2021Review
- The transcription regulator BrsR serves as a network hub of natural competence protein-protein interactions inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Threshold regulation and stochasticity from the MecA/ClpCP proteolytic system in Streptococcus mutans competence.Molecular microbiology · 2018Article
- Deficiency of MecA inFrontiers in microbiology · 2018Article
- Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2.Applied and environmental microbiology · 2017Article
- Origins of heterogeneity in Streptococcus mutans competence: interpreting an environment-sensitive signaling pathway.Physical biology · 2017Article
- Stress Physiology of Lactic Acid Bacteria.Microbiology and molecular biology reviews : MMBR · 2016Review
- Temporal Regulation of the Transformasome and Competence Development inFrontiers in microbiology · 2016Article
- Article
- A unique open reading frame within the comX gene of Streptococcus mutans regulates genetic competence and oxidative stress tolerance.Molecular microbiology · 2015Article
- Modeling of the ComRS Signaling Pathway Reveals the Limiting Factors Controlling Competence in Streptococcus thermophilus.Frontiers in microbiology · 2015Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Competence for natural DNA transformation is a tightly controlled developmental process in streptococci. In mutans and salivarius species, the abundance of the central competence regulator σ(X) is regulated at two levels: transcriptional, by the ComRS signaling system via the σ(X)/ComX/SigX-inducing peptide (XIP), and posttranscriptional, by the adaptor protein MecA and its associated Clp ATPase, ClpC. In this study, we further investigated the mechanism and function of the MecA-ClpC control system in the salivarius species Streptococcus thermophilus. Using in vitro approaches, we showed that MecA specifically interacts with both σ(X) and ClpC, suggesting the formation of a ternary σ(X)-MecA-ClpC complex. Moreover, we demonstrated that MecA ultimately targets σ(X) for its degradation by the ClpCP protease in an ATP-dependent manner. We also identify a short sequence (18 amino acids) in the N-terminal domain of σ(X) as essential for the interaction with MecA and subsequent σ(X) degradation. Finally, increased transformability of a MecA-deficient strain in the presence of subinducing XIP concentrations suggests that the MecA-ClpCP proteolytic complex acts as an additional locking device to prevent competence under inappropriate conditions. A model of the interplay between ComRS and MecA-ClpCP in the control of σ(X) activity is proposed.
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