ArticleJournal of bacteriology2014
Control of gene expression at a bacterial leader RNA, the agn43 gene encoding outer membrane protein Ag43 of Escherichia coli.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Multiscale regulation of nutrient stress responses in Escherichia coli from chromatin structure to small regulatory RNAs.Nucleic acids research · 2025Article
- Comparative genomics of Shiga toxin-producingFrontiers in veterinary science · 2025Article
- Extraintestinal PathogenicPathogens (Basel, Switzerland) · 2021Review
- Phenotypic and Genetic Determination of Biofilm Formation in Heat ResistantMicroorganisms · 2021Article
- NfiS, a species-specific regulatory noncoding RNA of Pseudomonas stutzeri, enhances oxidative stress tolerance in Escherichia coli.AMB Express · 2019Article
- Molecular characterization of enterotoxigenicVeterinary research forum : an international quarterly journal · 2019Article
- Virulence factors, prevalence and potential transmission of extraintestinal pathogenicGut pathogens · 2019Review
- Inactivation of Transcriptional Regulators during Within-Household Evolution of Escherichia coli.Journal of bacteriology · 2017Article
- Zinc oxide nanoparticle reduced biofilm formation and antigen 43 expressions in uropathogenicIranian journal of basic medical sciences · 2017Article
- Landscape of RNA polyadenylation in E. coli.Nucleic acids research · 2017Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The family of agn alleles in Escherichia coli pathovars encodes autotransporters that have been implicated in biofilm formation, autoaggregation, and attachment to cells. The alleles all have long leader RNAs preceding the Ag43 translation initiation codon. Here we present an analysis of the agn43 leader RNA from E. coli K-12. We demonstrate the presence of a rho-independent transcription terminator just 28 bp upstream of the main translation start codon and show that it is functional in vitro. Our data indicate that an as-yet-unknown mechanism of antitermination of transcription must be operative in earlier phases of growth. However, as bacterial cell cultures mature, progressively fewer transcripts are able to bypass this terminator. In the K-12 leader sequence, two in-frame translation initiation codons have been identified, one upstream and the other downstream of the transcription terminator. For optimal agn43 expression, both codons need to be present. Translation from the upstream start codon leads to increased downstream agn43 expression. Our findings have revealed two novel modes of regulation of agn43 expression in the leader RNA in addition to the previously well-characterized regulation of phase variation at the agn43 promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.