ArticlePloS one2014
High producer haplotype (CAG) of -863C/A, -308G/A and -238G/A polymorphisms in the promoter region of TNF-α gene associate with enhanced apoptosis of lymphocytes in HIV-1 subtype C infected individuals from North India.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 42 citations in OpenAlex.
- Hematological findings and complications of COVID-19.American journal of hematology · 2020Pooled it
- Factors associated with disease severity and mortality among patients with COVID-19: A systematic review and meta-analysis.PloS one · 2020Pooled it
- Evolution of Peritoneal Dialysis-Associated Peritonitis: Pathogen, Antibiotic Resistance, and the Impact of Lymphocyte Count on Treatment Outcomes.Infection and drug resistance · 2024Article
- Multi-omics in HIV: searching insights to understand immunological non-response in PLHIV.Frontiers in immunology · 2023Review
- The Dynamics of the Neutrophil-to-Lymphocyte and Platelet-to-Lymphocyte Ratios Predict Progression to Septic Shock and Death in Patients with Prolonged Intensive Care Unit Stay.Medicina (Kaunas, Lithuania) · 2022Article
- Clinical and Laboratory Findings by Serum Vitamin D Levels in Children with COVID-19.The Eurasian journal of medicine · 2022Article
- Oral Lesions Associated with COVID-19 and the Participation of the Buccal Cavity as a Key Player for Establishment of Immunity against SARS-CoV-2.International journal of environmental research and public health · 2022Review
- The TNF-α-308G/A Gene Polymorphism and Serum TNF-α Levels in Women With Preeclampsia.Journal of family & reproductive health · 2022Article
- Biomarkers during COVID-19: Mechanisms of Change and Implications for Patient Outcomes.Diagnostics (Basel, Switzerland) · 2022Review
- COVID-19: systemic pathology and its implications for therapy.International journal of biological sciences · 2022Review
- Late-onset hematological complications post COVID-19: An emerging medical problem for the hematologist.American journal of hematology · 2022Review
- Thrombosis and lymphocyte subsets of COVID-19 omicron BA.2 variant patients with cancer.Frontiers in oncology · 2022Article
- Analysis of laboratory blood parameter results for patients diagnosed with COVID-19, from all ethnic group populations: A single centre study.International journal of laboratory hematology · 2021Article
- Detection of SARS-CoV-2 virus using an alternative molecular method and evaluation of biochemical, hematological, inflammatory, and oxidative stress in healthcare professionals.Microbial pathogenesis · 2021Article
- Cell Population Data and Serum Polyclonal Immunoglobulin Free Light Chains in the Assessment of COVID-19 Severity.Viruses · 2021Article
- Hematological manifestations of SARS-CoV-2 in children.Pediatric blood & cancer · 2020Review
- Organ-specific manifestations of COVID-19 infection.Clinical and experimental medicine · 2020Review
- Inflammation and thrombosis in patients with COVID-19: A prothrombotic and inflammatory disease caused by SARS coronavirus-2.Anatolian journal of cardiology · 2020Review
- Associations of procalcitonin, C-reaction protein and neutrophil-to-lymphocyte ratio with mortality in hospitalized COVID-19 patients in China.Scientific reports · 2020Article
- Signature genes associated with immunological non-responsiveness to anti-retroviral therapy in HIV-1 subtype-c infection.PloS one · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe natural history of HIV-1 infection and its progression towards AIDS vary considerably among individuals. Host genetic factors may be one of the possible reasons for variable HIV-1 disease progression. Single nucleotide polymorphisms (SNPs) in the promoter region of TNF-α gene can influence its production. The aim of the present study was to determine the association of functional TNF-α SNPs and its associated parameters related to apoptosis that may influence the rate of HIV-1 disease progression.
methodsTherapy naive, 100 HIV slow progressors (SPs), 100 HIV fast progressors (FPs), 50 HIV exposed but seronegative individuals (ESNs) and 260 healthy controls from same ethnic origin were recruited. Genotyping of TNF-α variants (-863C/A, -308G/A and -238G/A) was done using PCR-RFLP. CD4 counts were determined by flow cytometry. Plasma viral load was estimated by COBAS AMPLICOR HIV-1 monitor test. Plasma TNF-α concentration was estimated by Human CBA Th1/Th2 cytokine kit. The lymphocyte mitochondrial membrane potential was measured by JC-1 dye by flow cytometry.
resultsGenotype and allele frequency of TNF-α -238G/A and -863C/A was not significantly different in HIV-1-infected patients when compared to controls, while that of TNF-α -308G/A variant (high TNF-α producer) was significantly higher in FPs compared to SPs (p<0.01, OR = 3.43). Haplotype analyses also showed that carriers of high TNF-α producing haplotype CAG was significantly more common among FPs compared to SPs (p<0.01, OR = 3). The circulating TNF-α levels in blood also correlated well with genotypes. The lymphocyte mitochondrial membrane potential of FPs having CAG haplotype was significantly low as compared to wild type (CGG) haplotype (417±22 vs 571±28, p<0.01).
conclusionHigh producer haplotype, CAG of TNF-α gene associates with enhanced apoptosis of lymphocytes in HIV-1 infected individuals, hence faster progression to AIDS. However, further functional studies are needed to confirm this association and this knowledge may help clinicians to better understand the disease outcome.
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