Evidence map›Paper›PMID 24836948›Full record

ArticleJPEN. Journal of parenteral and enteral nutrition2015

Glutamine Improves Innate Immunity and Prevents Bacterial Enteroinvasion During Parenteral Nutrition.

Xinying Wang, Joseph F Pierre, Aaron F Heneghan, Rebecca A Busch, Kenneth A Kudsk

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JPEN. Journal of parenteral and enteral nutrition, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed, 2 pooled it
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 2 syntheses or guidelines pooled it, 35 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Xinying WangDepartment of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin Department of Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
Joseph F PierreDepartment of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin Veterans Administration Surgical Services, William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin.
Aaron F HeneghanDepartment of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin Veterans Administration Surgical Services, William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin.
Rebecca A BuschDepartment of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
Kenneth A KudskDepartment of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin Veterans Administration Surgical Services, William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin kudsk@surgery.wisc.edu.
University of Wisconsin–Madison · USNanjing General Hospital of Nanjing Military Command · CNWilliam S. Middleton Memorial Veterans Hospital · US

Funding

UW Surgical Oncology Research Training Program (UW-SORT)T32CA090217 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI Muhammed Murtaza, Heather B Neuman · 2001 to 2026
$8.3M
Route of Nutrition and Enteric Nervous System Effects on Innate Mucosal DefenseI01BX001672 · VA · WM S. MIDDLETON MEMORIAL VETERANS HOSP · PI KUDSK, KENNETH A · 2012 to 2015
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BLRD VA I01 BX001672NCI NIH HHS T32 CA090217NCI NIH HHS T32CA090217-13
6 · The paper itself

Abstract

backgroundPatients receiving parenteral nutrition (PN) are at increased risk of infectious complications compared with enteral feeding, which is in part explained by impaired mucosal immune function during PN. Adding glutamine (GLN) to PN has improved outcome in some clinical patient groups. Although GLN improves acquired mucosal immunity, its effect on innate mucosal immunity (defensins, mucus, lysozymes) has not been investigated.

methodsForty-eight hours following venous cannulation, male Institute of Cancer Research mice were randomized to chow (n = 10), PN (n = 12), or PN + GLN (n = 13) for 5 days. Small intestine tissue and luminal fluid were collected for mucin 2 (MUC2), lysozyme, cryptdin 4 analysis, and luminal interleukin (IL)-4, IL-10, and IL-13 level measurement. Tissue was also harvested for ex vivo intestinal segment culture to assess tissue susceptibility to enteroinvasive Escherichia coli.

resultsIn both luminal and tissue samples, PN reduced MUC2 and lysozyme (P < .0001, respectively) compared with chow, whereas GLN addition increased MUC2 and lysozyme (luminal, P < .05; tissue, P < .0001, respectively) compared with PN alone. PN significantly suppressed cryptdin 4 expression, while GLN supplementation significantly enhanced expression. IL-4, IL-10, and IL-13 decreased significantly with PN compared with chow, whereas GLN significantly increased these cytokines compared with PN. Functionally, bacterial invasion increased with PN compared with chow (P < .05), while GLN significantly decreased enteroinvasion to chow levels (P < .05).

conclusionsGLN-supplemented PN improves innate immunity and resistance to bacterial mucosal invasion lost with PN alone. This work confirms a clinical rationale for providing glutamine for the protection of the intestinal mucosa.

Indexed as

Parenteral Nutritionalpha-DefensinsAnimalsEscherichia coliEscherichia coli InfectionsGene Expression RegulationGlutamineImmunity, InnateImmunity, MucosalInterleukin-10Interleukin-13Interleukin-4Intestinal MucosaIntestinesMaleMicealpha-Defensinscryptdin 4, mouseGlutamineInterleukin-10Interleukin-13Interleukin-4Muc2 protein, mouseMucin-2cryptdin 4glutamineinnate immunityintestinal mucosalysozymeparenteral nutrition

Identifiers

PMID24836948
PMCPMC4233200
OpenAlexW2026393795

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.