ArticlePloS one2014
Characterization of the RnfB and RnfG subunits of the Rnf complex from the archaeon Methanosarcina acetivorans.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 36 citations in OpenAlex.
- The RNF/NQR redox pumps: a versatile system for energy transduction in bacteria and archaea.Applied microbiology and biotechnology · 2025Review
- Architecture of the RNF1 complex that drives biological nitrogen fixation.Nature chemical biology · 2024Article
- Purification and structural characterization of the NaNature communications · 2022Article
- Required Gene Set for Autotrophic Growth ofApplied and environmental microbiology · 2022Article
- DeconstructingProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Improvement of Direct Interspecies Electron TransferFrontiers in microbiology · 2022Review
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- Structure and function of an unusual flavodoxin from the domainProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the GenusMicrobiology and molecular biology reviews : MMBR · 2019Review
- A Membrane-Bound Cytochrome EnablesmBio · 2019Article
- The Membrane-Bound C Subunit of Reductive Dehalogenases: Topology Analysis and Reconstitution of the FMN-Binding Domain of PceC.Frontiers in microbiology · 2018Article
- Physiological Evidence for Isopotential Tunneling in the Electron Transport Chain of Methane-Producing Archaea.Applied and environmental microbiology · 2017Article
- Functional Role of MrpA in the MrpABCDEFG Na+/H+ Antiporter Complex from the Archaeon Methanosarcina acetivorans.Journal of bacteriology · 2017Article
- Assessing methanotrophy and carbon fixation for biofuel production by Methanosarcina acetivorans.Microbial cell factories · 2016Article
- Article
- Metabolic response of Clostridium ljungdahlii to oxygen exposure.Applied and environmental microbiology · 2015Article
- Rerouting Cellular Electron Flux To Increase the Rate of Biological Methane Production.Applied and environmental microbiology · 2015Article
- Acetate Metabolism in Anaerobes from the Domain Archaea.Life (Basel, Switzerland) · 2015Review
- Complete topology of the RNF complex from Vibrio cholerae.Biochemistry · 2015Article
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4 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Rnf complexes are redox-driven ion pumps identified in diverse species from the domains Bacteria and Archaea, biochemical characterizations of which are reported for two species from the domain Bacteria. Here, we present characterizations of the redox-active subunits RnfG and RnfB from the Rnf complex of Methanosarcina acetivorans, an acetate-utilizing methane-producing species from the domain Archaea. The purified RnfG subunit produced in Escherichia coli fluoresced in SDS-PAGE gels under UV illumination and showed a UV-visible spectrum typical of flavoproteins. The Thr166Gly variant of RnfG was colorless and failed to fluoresce under UV illumination confirming a role for Thr166 in binding FMN. Redox titration of holo-RnfG revealed a midpoint potential of -129 mV for FMN with n = 2. The overproduced RnfG was primarily localized to the membrane of E. coli and the sequence contained a transmembrane helix. A topological analysis combining reporter protein fusion and computer predictions indicated that the C-terminal domain containing FMN is located on the outer aspect of the cytoplasmic membrane. The purified RnfB subunit produced in E. coli showed a UV-visible spectrum typical of iron-sulfur proteins. The EPR spectra of reduced RnfB featured a broad spectral shape with g values (2.06, 1.94, 1.90, 1.88) characteristic of magnetically coupled 3Fe-4S and 4Fe-4S clusters in close agreement with the iron and acid-labile sulfur content. The ferredoxin specific to the aceticlastic pathway served as an electron donor to RnfB suggesting this subunit is the entry point of electrons to the Rnf complex. The results advance an understanding of the organization and biochemical properties of the Rnf complex and lay a foundation for further understanding the overall mechanism in the pathway of methane formation from acetate.
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