ArticleDiabetes2014
Increased DNA methyltransferase 3b (Dnmt3b)-mediated CpG island methylation stimulated by oxidative stress inhibits expression of a gene required for neural tube and neural crest development in diabetic pregnancy.
Article in Diabetes, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 72 citations in OpenAlex.
- Prenatal exposure to gestational diabetes mellitus increases developmental defects in the enamel of offspring.PloS one · 2019Trial
- H19 inhibition orchestrates DNMT3B-dependent epigenetic reprogramming of glycolytic enzymes in the liver during diabetes.Cell communication and signaling : CCS · 2026Article
- The silent threat: PMArchives of toxicology · 2026Review
- A Review of the Impact of Gestational Diabetes on Fetal Brain Development: An Update on Neurosonographic Markers During the Last Decade.Life (Basel, Switzerland) · 2025Review
- Association of DNA Methylation with Infant Birth Weight in Women with Gestational Diabetes.Metabolites · 2024Review
- Epigenetic implications in maternal diabetes and metabolic syndrome-associated risk of orofacial clefts.Birth defects research · 2023Review
- Epigenetic modifier alpha-ketoglutarate modulates aberrant gene body methylation and hydroxymethylation marks in diabetic heart.Epigenetics & chromatin · 2023Article
- An exploratory study of maternal diabetes and offspring use of dental services-Northern Ireland national cohort study.BDJ open · 2023Article
- Inheritance of perturbed methylation and metabolism caused by uterine malnutrition via oocytes.BMC biology · 2023Article
- The interplay of inflammation, exosomes and CaCardiovascular diabetology · 2023Review
- Functional cargos of exosomes derived from Flk-1Communications biology · 2022Article
- Abnormal methylation caused by folic acid deficiency in neural tube defects.Open life sciences · 2022Review
- Epigenetic analysis in placentas from sickle cell disease patients reveals a hypermethylation profile.PloS one · 2022Article
- Prevention of Teratogenesis in Pregnancies of Obese Rats by Vitamin E Supplementation.Antioxidants (Basel, Switzerland) · 2021Article
- Functional Interplay between Methyltransferases and Inflammasomes in Inflammatory Responses and Diseases.International journal of molecular sciences · 2021Review
- Diabetes, Oxidative Stress, and DNA Damage Modulate Cranial Neural Crest Cell Development and the Phenotype Variability of Craniofacial Disorders.Frontiers in cell and developmental biology · 2021Review
- Molecular Mechanisms and Epigenetic Regulation in Diabetic Cardiomyopathy.Frontiers in cardiovascular medicine · 2021Review
- Mechanisms of Congenital Malformations in Pregnancies with Pre-existing Diabetes.Current diabetes reports · 2020Review
- Aberrant DNA methylation as a diagnostic biomarker of diabetic embryopathy.Genetics in medicine : official journal of the American College of Medical Genetics · 2019Article
- The relationship among occupational irradiation, DNA methylation status, and oxidative damage in interventional physicians.Medicine · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Previous studies have shown that diabetic embryopathy results from impaired expression of genes that are required for formation of embryonic structures. We have focused on Pax3, a gene that is expressed in embryonic neuroepithelium and is required for neural tube closure. Pax3 expression is inhibited in embryos of diabetic mice due to hyperglycemia-induced oxidative stress. DNA methylation silences developmentally expressed genes before differentiation. We hypothesized that hypomethylation of Pax3 upon neuroepithelial differentiation may be inhibited by hyperglycemia-induced oxidative stress. We tested this using embryos of pregnant hyperglycemic mice and mouse embryonic stem cells (ESC). Methylation of a Pax3 CpG island decreased upon neurulation of embryos and formation of neuronal precursors from ESC. In ESC, this was inhibited by oxidative stress. Use of short hairpin RNA in ESC demonstrated that DNA methyltransferase 3b (Dnmt3b) was responsible for methylation and silencing of Pax3 before differentiation and by oxidative stress. Although expression of Dnmt3b was not affected by oxidative stress, DNA methyltransferase activity was increased. These results indicate that hyperglycemia-induced oxidative stress stimulates Dnmt3b activity, thereby inhibiting chromatin modifications necessary for induction of Pax3 expression during neurulation and thus providing a molecular mechanism for defects caused by Pax3 insufficiency in diabetic pregnancy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.