ArticleNature protocols2014
DNA origami-based standards for quantitative fluorescence microscopy.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed.
- pH-Driven Actuation of Unmodified DNA Origami Rectangles.Angewandte Chemie (International ed. in English) · 2026Article
- Nanorulers for Super-Resolution Microscopy.Chembiochem : a European journal of chemical biology · 2026Review
- DNA-Enzyme Hybrid Nanostructures: Functional Materials to Modulate Enzymatic Activity.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- NanorulerQA: quantitative quality analysis of dual-color DNA nanorulers via single-molecule photobleaching step counting and spatio-temporal colocalization.Biomedical optics express · 2026Article
- Reactive oxygen species-resistant ultrastable super-resolution DNA framework dots.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Multiplexed and high-bandwidth DNA computing circuits with superresolution DNA origami displays.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Spectral Flow Cytometry: The Current State and Future of the Technology.International journal of molecular sciences · 2025Review
- Radiation and DNA Origami Nanotechnology: Probing Structural Integrity at the Nanoscale.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Review
- 2D Titanium Carbide MXene and Single-Molecule Fluorescence: Distance-Dependent Nonradiative Energy Transfer and Leaflet-Resolved Dye Sensing in Lipid Bilayers.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Single-molecule detection and super-resolution imaging with a portable and adaptable 3D-printed microscopy platform (Brick-MIC).Science advances · 2024Article
- MultiMatch: geometry-informed colocalization in multi-color super-resolution microscopy.Communications biology · 2024Article
- Visualization of the hepatic and renal cell uptake and trafficking of tetrahedral DNA origami in tumour.Cell proliferation · 2024Article
- Single-molecule detection and super-resolution imaging with a portable and adaptable 3D-printed microscopy platform (Brick-MIC).bioRxiv : the preprint server for biology · 2024Article
- Fluorophore multimerization on a PEG backbone as a concept for signal amplification and lifetime modulation.Scientific reports · 2024Article
- Dominant Analytical Techniques in DNA Nanotechnology for Various Applications.Analytical chemistry · 2024Article
- Quantitative Single-Molecule Localization Microscopy.Annual review of biophysics · 2023Review
- Expansion-enhanced super-resolution radial fluctuations enable nanoscale molecular profiling of pathology specimens.Nature nanotechnology · 2023Article
- Towards Unbiased Fluorophore Counting in Superresolution Fluorescence Microscopy.Nanomaterials (Basel, Switzerland) · 2023Article
- Gradient-mixing LEGO robots for purifying DNA origami nanostructures of multiple components by rate-zonal centrifugation.PloS one · 2023Article
- Synthesizing the biochemical and semiconductor worlds:Nanoscale · 2022Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Validating and testing a fluorescence microscope or a microscopy method requires defined samples that can be used as standards. DNA origami is a new tool that provides a framework to place defined numbers of small molecules such as fluorescent dyes or proteins in a programmed geometry with nanometer precision. The flexibility and versatility in the design of DNA origami microscopy standards makes them ideally suited for the broad variety of emerging super-resolution microscopy methods. As DNA origami structures are durable and portable, they can become a universally available specimen to check the everyday functionality of a microscope. The standards are immobilized on a glass slide, and they can be imaged without further preparation and can be stored for up to 6 months. We describe a detailed protocol for the design, production and use of DNA origami microscopy standards, and we introduce a DNA origami rectangle, bundles and a nanopillar as fluorescent nanoscopic rulers. The protocol provides procedures for the design and realization of fluorescent marks on DNA origami structures, their production and purification, quality control, handling, immobilization, measurement and data analysis. The procedure can be completed in 1-2 d.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.