ArticleNature protocols2014
Engineering complex biological systems in bacteria through recombinase-assisted genome engineering.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- High-throughput editing boosts the construction of next-generation microbial cell factories.Synthetic and systems biotechnology · 2026Review
- EngineeringACS synthetic biology · 2026Article
- Bacterial live therapeutics for human diseases.Molecular systems biology · 2024Review
- A Framework for the Systematic Selection of Biosensor Chassis for Environmental Synthetic Biology.ACS synthetic biology · 2022Review
- Creating custom synthetic genomes in Escherichia coli with REXER and GENESIS.Nature protocols · 2021Article
- CRAGE enables rapid activation of biosynthetic gene clusters in undomesticated bacteria.Nature microbiology · 2019Article
- Genetic platforms for heterologous expression of microbial natural products.Natural product reports · 2019Review
- An integrated workflow for phenazine-modifying enzyme characterization.Journal of industrial microbiology & biotechnology · 2018Article
- Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases.ACS synthetic biology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we describe an advanced paradigm for the design, construction and stable implementation of complex biological systems in microbial organisms. This engineering strategy was previously applied to the development of an Escherichia coli-based platform, which enabled the use of brown macroalgae as a feedstock for the production of biofuels and renewable chemicals. In this approach, functional genetic modules are first designed in silico and constructed on a bacterial artificial chromosome (BAC) by using a recombineering-based inchworm extension technique. Stable integration into the recipient chromosome is then mediated through the use of recombinase-assisted genome engineering (RAGE). The flexibility, simplicity and speed of this method enable a comprehensive optimization of several different parameters, including module configuration, strain background, integration locus, gene copy number and intermodule compatibility. This paradigm therefore has the potential to markedly expedite most strain-engineering endeavors. Once a biological system has been designed and constructed on a BAC, its implementation and optimization in a recipient host can be carried out in as little as 1 week.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.