ArticleMolecular genetics and genomics : MGG2014
GhAGL15s, preferentially expressed during somatic embryogenesis, promote embryogenic callus formation in cotton (Gossypium hirsutum L.).
Article in Molecular genetics and genomics : MGG, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- AGL15 Promotion of Somatic Embryogenesis: Role and Molecular Mechanism.Frontiers in plant science · 2022Pooled it
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- Appreciating animal induced pluripotent stem cells to shape plant cell reprogramming strategies.Journal of experimental botany · 2024Review
- Regulatory mechanisms of the LBD40 transcription factor inPlant direct · 2023Article
- Promoting genotype-independent plant transformation by manipulating developmental regulatory genes and/or using nanoparticles.Plant cell reports · 2023Review
- Multifaceted roles of transcription factors during plant embryogenesis.Frontiers in plant science · 2023Review
- Revisiting AGAMOUS-LIKE15, a Key Somatic Embryogenesis Regulator, Using Next Generation Sequencing Analysis inInternational journal of molecular sciences · 2022Article
- The MADS-domain factor AGAMOUS-Like18 promotes somatic embryogenesis.Plant physiology · 2022Article
- Genotype-independent plant transformation.Horticulture research · 2022Article
- Integrated Proteomic and Metabolomic Analyses Provide Insights Into Acquisition of Embryogenic Ability inFrontiers in plant science · 2022Article
- Examination of the Transcriptional Response toBiology · 2021Article
- Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis.International journal of molecular sciences · 2021Review
- Transcriptomes analysis reveals novel insight into the molecular mechanisms of somatic embryogenesis in Hevea brasiliensis.BMC genomics · 2021Article
- Embryogenic Calli Induction and Salt Stress Response Revealed by RNA-Seq in Diploid Wild SpeciesFrontiers in plant science · 2021Article
- Genetic activity during early plant embryogenesis.The Biochemical journal · 2020Review
- Dynamic TMT-Based Quantitative Proteomics Analysis of Critical Initiation Process of Totipotency during Cotton Somatic Embryogenesis Transdifferentiation.International journal of molecular sciences · 2019Article
- Using Morphogenic Genes to Improve Recovery and Regeneration of Transgenic Plants.Plants (Basel, Switzerland) · 2019Review
- Transcriptome Profiling Predicts New Genes to Promote Maize Callus Formation and Transformation.Frontiers in plant science · 2019Article
- Genome-Wide Analysis of the NF-YB Gene Family in Gossypium hirsutum L. and Characterization of the Role of GhDNF-YB22 in Embryogenesis.International journal of molecular sciences · 2018Article
- iTRAQ-based comparative proteomic analysis provides insights into somatic embryogenesis in Gossypium hirsutum L.Plant molecular biology · 2018Article
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8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Somatic embryogenesis is a useful tool for gene transfer and propagation of plants. AGAMOUS-LIKE15 (AGL15) promotes somatic embryogenesis in many plant species. In this study, three homologous AGL15 genes were isolated from Gossypium hirsutum L., namely GhAGL15-1, GhAGL15-3, and GhAGL15-4. Their putative proteins contained a highly conserved MADS-box DNA-binding domain and a less conserved K domain. Phylogenetic analysis suggested that the three GhAGL15s clustered most closely with AGL15 proteins in other plants. Subcellular location analyses revealed that three GhAGL15s were localized in the nucleus. Furthermore, their expression levels increased following embryogenic callus induction, but sharply decreased during the embryoid stage. GhAGL15-1 and GhAGL15-3 were significantly induced by 2,4-D and kinetin, whereas GhAGL15-4 was only responsive to 2,4-D treatment. Over-expression of the three GhAGL15s in cotton callus improved callus quality and significantly increased the embryogenic callus formation rate, while GhAGL15-4 had the highest positive effect on the embryogenic callus formation rate (an increase from 38.1 to 65.2%). These results suggest that over-expression of GhAGL15s enhances embryogenic potential of transgenic calli. Therefore, spatiotemporal manipulation of GhAGL15s expression may prove valuable in improving cotton transformation efficiency.
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