ArticleParasitology research2014
The effect of environmental and physiological conditions on excystation of Acanthamoeba castellanii belonging to the T4 genotype.
Article in Parasitology research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.
- Orthokeratology lens-relatedThe Journal of international medical research · 2021Pooled it
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- Incidence of Acanthamoeba Keratitis in Switzerland.Microorganisms · 2025Article
- Prevalence of free-living amoebae in five rivers associated with high human activity in Tehran province, Iran.Journal of water and health · 2025Article
- Identification of essential genes for Acanthamoeba castellanii excystation during encystation and excystation.Parasites, hosts and diseases · 2024Article
- Assessing PCR-Positive Acanthamoeba Keratitis-A Retrospective Chart Review.Microorganisms · 2024Article
- Application of Dendrimers for Treating Parasitic Diseases.Pharmaceutics · 2021Review
- Evaluation of Acanthamoeba keratitis cases in a tertiary medical care centre over 21 years.Scientific reports · 2021Article
- Article
- Gold Nanoparticle Conjugation Enhances the Antiacanthamoebic Effects of Chlorhexidine.Antimicrobial agents and chemotherapy · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Excystation in Acanthamoeba is an important property for the onset of infection as well as infection recurrence, post-treatment. The overall aim of this study was to determine the effects of several environmental and physiological parameters on excystation in Acanthamoeba castellanii belonging to the T4 genotype. Cysts were prepared by inoculating A. castellanii trophozoites on non-nutrient agar plates for up to 2 weeks. To determine the effects of various conditions on excystation, A. castellanii cysts were inoculated in growth medium i.e. PYG and incubated at varying temperatures (4-40 °C), various pHs (4-9), artificial light/dark cycles and 5% of CO2. Optimum excystation was observed when cysts were incubated at 30 °C in growth medium at neutral pH. Extremes of temperature and pH reduced excystation, while light/dark cycles had no effect on excystation of A. castellanii. On the other hand, 5% of CO2 enhanced excystation and growth of excysting amoebae. To determine the effect of serum on A. castellanii excystation, assays were performed in the presence of varying concentrations of heat-inactivated foetal bovine serum (FBS) (5-100%). The results revealed that FBS promoted excystation. The involvement of G proteins in excystation was also determined. Using propranolol hydrochloride, a G protein inhibitor, the results revealed that G proteins play a role in A. castellanii differentiation. Furthermore, organic solvents (methanol/ethanol) completely blocked excystation. None of the aforementioned conditions had any effect on the viability of A. castellanii. A complete understanding of excystation in A. castellanii will be of value to counter infection recurrence.
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