ArticleBiology2013
Isolation and characterization of bacteria from ancient siberian permafrost sediment.
Article in Biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 68 citations in OpenAlex.
- Optimization and Characterization of Bioactive Metabolites from Cave-DerivedBiomolecules · 2025Article
- Pandemic Events Caused by Bacteria Throughout Human History and the Risks of Antimicrobial Resistance Today.Microorganisms · 2025Review
- The Petri dish under the ice: permafrost pathogens and their impact on global healthcare and antibiotic resistance.Annals of medicine and surgery (2012) · 2024Review
- Taxonomic diversity and environmental tolerance of cultivable extremophilic bacteria from a high-altitude meltwater pond on Ojos del Salado (Chile).Biologia futura · 2024Article
- Earth to Mars: A Protocol for Characterizing Permafrost in the Context of Climate Change as an Analog for Extraplanetary Exploration.Astrobiology · 2023Article
- Complete Genome Analysis ofMicroorganisms · 2023Article
- Microbial life in 25-m-deep boreholes in ancient permafrost illuminated by metagenomics.Environmental microbiome · 2023Article
- Prevalence and abundance of antibiotic-resistant genes in culturable bacteria inhabiting a non-polar passu glacier, karakorum mountains range, Pakistan.World journal of microbiology & biotechnology · 2023Article
- A Defined Medium for Cultivation and Exometabolite Profiling of Soil Bacteria.Frontiers in microbiology · 2022Article
- Genome Analysis ofBiology · 2021Article
- Bacterial community characterization of Batura Glacier in the Karakoram Range of Pakistan.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Article
- Culturing Ancient Bacteria Carrying Resistance Genes from Permafrost and Comparative Genomics with Modern Isolates.Microorganisms · 2020Article
- AMicroorganisms · 2020Article
- Prokaryotic community in Pleistocene ice wedges of Mammoth Mountain.Extremophiles : life under extreme conditions · 2020Article
- Subtercola vilae sp. nov., a novel actinobacterium from an extremely high-altitude cold volcano lake in Chile.Antonie van Leeuwenhoek · 2018Article
- Novel actinomycin group compound from newly isolated Streptomyces sp. RAB12: isolation, characterization, and evaluation of antimicrobial potential.Applied microbiology and biotechnology · 2018Article
- Prokaryotic Abundance and Activity in Permafrost of the Northern Victoria Land and Upper Victoria Valley (Antarctica).Microbial ecology · 2017Article
- Ancient permafrost staphylococci carry antibiotic resistance genes.Microbial ecology in health and disease · 2017Article
- Recovery of metallo-tolerant and antibiotic resistant psychrophilic bacteria from Siachen glacier, Pakistan.PloS one · 2017Article
- Is the ancient permafrost bacteria able to keep DNA stable?Journal of genetics · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we isolated and characterized bacterial strains from ancient (Neogene) permafrost sediment that was permanently frozen for 3.5 million years. The sampling site was located at Mammoth Mountain in the Aldan river valley in Central Yakutia in Eastern Siberia. Analysis of phospolipid fatty acids (PLFA) demonstrated the dominance of bacteria over fungi; the analysis of fatty acids specific for Gram-positive and Gram-negative bacteria revealed an approximately twofold higher amount of Gram-negative bacteria compared to Gram-positive bacteria. Direct microbial counts after natural permafrost enrichment showed the presence of (4.7 ± 1.5) × 108 cells g-1 sediment dry mass. Viable heterotrophic bacteria were found at 0 °C, 10 °C and 25 °C, but not at 37 °C. Spore-forming bacteria were not detected. Numbers of viable fungi were low and were only detected at 0 °C and 10 °C. Selected culturable bacterial isolates were identified as representatives of Arthrobacter phenanthrenivorans, Subtercola frigoramans and Glaciimonas immobilis. Representatives of each of these species were characterized with regard to their growth temperature range, their ability to grow on different media, to produce enzymes, to grow in the presence of NaCl, antibiotics, and heavy metals, and to degrade hydrocarbons. All strains could grow at -5 °C; the upper temperature limit for growth in liquid culture was 25 °C or 30 °C. Sensitivity to rich media, antibiotics, heavy metals, and salt increased when temperature decreased (20 °C > 10 °C > 1 °C). In spite of the ligninolytic activity of some strains, no biodegradation activity was detected.
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Registered trials
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