ArticlePLoS genetics2014
Intron retention in the 5'UTR of the novel ZIF2 transporter enhances translation to promote zinc tolerance in arabidopsis.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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51 citing papers in PubMed, 123 citations in OpenAlex.
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- Untranslated yet indispensable-UTRs act as key regulators in the environmental control of gene expression.Journal of experimental botany · 2024Review
- Importance of pre-mRNA splicing and its study tools in plants.Advanced biotechnology · 2024Review
- BRUTUS-LIKE (BTSL) E3 ligase-mediated fine-tuning of Fe regulation negatively affects Zn tolerance of Arabidopsis.Journal of experimental botany · 2023Article
- Mapping intron retention events contributing to complex traits using splice quantitative trait locus.Plant methods · 2023Article
- The Splicing Factor OsSCL26 Regulates Phosphorus Homeostasis in Rice.Plants (Basel, Switzerland) · 2023Article
- Genome-Wide Association Analysis for Hybrid Breeding in Wheat.International journal of molecular sciences · 2022Article
- OsTBP2.1, a TATA-Binding Protein, Alters the Ratio ofInternational journal of molecular sciences · 2022Article
- Mining of Potential Gene Resources for Breeding Nutritionally Improved Maize.Plants (Basel, Switzerland) · 2022Review
- Diverse Functions of Plant Zinc-Induced Facilitator-like Transporter for Their Emerging Roles in Crop Trait Enhancement.Plants (Basel, Switzerland) · 2021Review
- Low-molecular-weight ligands in plants: role in metal homeostasis and hyperaccumulation.Photosynthesis research · 2021Review
- Phosphorus Starvation- and Zinc Excess-InducedJournal of fungi (Basel, Switzerland) · 2021Article
- Normal, novel or none: versatile regulation from alternative splicing.Plant signaling & behavior · 2021Article
- Zinc toxicity in plants: a review.Planta · 2021Review
- Comparative transcriptome analyses revealed different heat stress responses in pigeonpea (Cajanus cajan) and its crop wild relatives.Plant cell reports · 2021Article
- Arabidopsis thaliana zinc accumulation in leaf trichomes is correlated with zinc concentration in leaves.Scientific reports · 2021Article
- Genome-Wide Identification and Characterization of Potato Long Non-coding RNAs Associated WithFrontiers in plant science · 2021Article
- Early Response to Dehydration Six-Like Transporter Family: Early Origin in Streptophytes and Evolution in Land Plants.Frontiers in plant science · 2021Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Root vacuolar sequestration is one of the best-conserved plant strategies to cope with heavy metal toxicity. Here we report that zinc (Zn) tolerance in Arabidopsis requires the action of a novel Major Facilitator Superfamily (MFS) transporter. We show that ZIF2 (Zinc-Induced Facilitator 2) localises primarily at the tonoplast of root cortical cells and is a functional transporter able to mediate Zn efflux when heterologously expressed in yeast. By affecting plant tissue partitioning of the metal ion, loss of ZIF2 function exacerbates plant sensitivity to excess Zn, while its overexpression enhances Zn tolerance. The ZIF2 gene is Zn-induced and an intron retention event in its 5'UTR generates two splice variants (ZIF2.1 and ZIF2.2) encoding the same protein. Importantly, high Zn favours production of the longer ZIF2.2 transcript, which compared to ZIF2.1 confers greater Zn tolerance to transgenic plants by promoting higher root Zn immobilization. We show that the retained intron in the ZIF2 5'UTR enhances translation in a Zn-responsive manner, markedly promoting ZIF2 protein expression under excess Zn. Moreover, Zn regulation of translation driven by the ZIF2.2 5'UTR depends largely on a predicted stable stem loop immediately upstream of the start codon that is lost in the ZIF2.1 5'UTR. Collectively, our findings indicate that alternative splicing controls the levels of a Zn-responsive mRNA variant of the ZIF2 transporter to enhance plant tolerance to the metal ion.
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