Evidence map›Paper›PMID 24832154›Full record

ArticleNature communications2014

An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging.

Dan Yu, William Clay Gustafson, Chun Han, Céline Lafaye, Marjolaine Noirclerc-Savoye, Woo-Ping Ge, Desiree A Thayer, Hai Huang, Thomas B Kornberg, Antoine Royant and 4 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.

0numbers the graph read from it
0cells of the map it votes in
93citing papers in PubMed
26.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

93 citing papers in PubMed, 164 citations in OpenAlex.

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  4. The near-infrared bacteriophytochrome-derived fluorescent protein PENELOPE enables RESOLFT superresolution microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2025
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  9. Phase separation of YAP-MAML2 differentially regulates the transcriptome.Proceedings of the National Academy of Sciences of the United States of America · 2024
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  15. Characterizing Fluorescence Properties of Turn-on RNA Aptamers.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  16. Conserved histidine and tyrosine determine spectral responses through the water network in Deinococcus radiodurans phytochrome.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2022
    Article
  17. Article
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  19. Review
  20. Review

33 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 2 countries.

Dan Yu1] Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94158, USA [2] Cardiovascular Research Institute, University of California, San Francisco, California 94158, USA.
William Clay GustafsonDepartment of Pediatrics, Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, California 94158, USA.
Chun Han1] Cardiovascular Research Institute, University of California, San Francisco, California 94158, USA [2] Department of Biophysics and Biochemistry, University of California, San Francisco, California 94158, USA [3] Department of Physiology, University of California, San Francisco, California 94158, USA.
Céline Lafaye1] Université Grenoble Alpes, Institut de Biologie Structurale (IBS), Grenoble F-38000, France [2] CNRS, IBS, Grenoble F-38000, France [3] CEA, DSV, IBS, Grenoble F-38000, France.
Marjolaine Noirclerc-Savoye1] Université Grenoble Alpes, Institut de Biologie Structurale (IBS), Grenoble F-38000, France [2] CNRS, IBS, Grenoble F-38000, France [3] CEA, DSV, IBS, Grenoble F-38000, France.
Woo-Ping Ge1] Cardiovascular Research Institute, University of California, San Francisco, California 94158, USA [2] Department of Biophysics and Biochemistry, University of California, San Francisco, California 94158, USA [3] Department of Physiology, University of California, San Francisco, California 94158, USA.
Desiree A Thayer1] Cardiovascular Research Institute, University of California, San Francisco, California 94158, USA [2] Department of Biophysics and Biochemistry, University of California, San Francisco, California 94158, USA [3] Department of Physiology, University of California, San Francisco, California 94158, USA.
Hai Huang1] Cardiovascular Research Institute, University of California, San Francisco, California 94158, USA [2] Department of Biophysics and Biochemistry, University of California, San Francisco, California 94158, USA.
Thomas B Kornberg1] Cardiovascular Research Institute, University of California, San Francisco, California 94158, USA [2] Department of Biophysics and Biochemistry, University of California, San Francisco, California 94158, USA.
Antoine Royant1] Université Grenoble Alpes, Institut de Biologie Structurale (IBS), Grenoble F-38000, France [2] CNRS, IBS, Grenoble F-38000, France [3] CEA, DSV, IBS, Grenoble F-38000, France [4] European Synchrotron Radiation Facility, Grenoble F-38043, France.
Lily Yeh Jan1] Cardiovascular Research Institute, University of California, San Francisco, California 94158, USA [2] Department of Biophysics and Biochemistry, University of California, San Francisco, California 94158, USA [3] Department of Physiology, University of California, San Francisco, California 94158, USA [4] Howard Hughes Medical Institute, University of California, San Francisco, California 94158, USA.
Yuh Nung Jan1] Cardiovascular Research Institute, University of California, San Francisco, California 94158, USA [2] Department of Biophysics and Biochemistry, University of California, San Francisco, California 94158, USA [3] Department of Physiology, University of California, San Francisco, California 94158, USA [4] Howard Hughes Medical Institute, University of California, San Francisco, California 94158, USA.
William A Weiss1] Department of Pediatrics, Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, California 94158, USA [2] Departments of Neurology and Neurosurgery, Brain Tumor Research Center, University of California, San Francisco, California 94158, USA.
Xiaokun Shu1] Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94158, USA [2] Cardiovascular Research Institute, University of California, San Francisco, California 94158, USA.
University of California, San Francisco · USCEA Grenoble · FRCentre National de la Recherche Scientifique · FRUniversité Grenoble Alpes · FR

Funding

MOLECULAR MECHANISMS IN DEVELOPMENTR01GM030637 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KORNBERG, THOMAS B. · 1985 to 2017
$6.8M
Research Training in Childhood CancerT32CA128583 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Eric Alejandro Sweet-Cordero · 2007 to 2026
$6.0M
The vascular niche in glioma propagation and resistance to therapyU54CA163155 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BERGERS, GABRIELE, WEAVER, VALERIE MARIE · 2011 to 2015
$4.4M
Local Proliferation of Glia and their Interaction with Blood VesselsR00NS073735 · NINDS · UT SOUTHWESTERN MEDICAL CENTER · PI GE, WOO-PING · 2014 to 2016
$731k
Blockade of p53 and Aurora A in Therapy Resistant NeuroblastomaK08NS079485 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GUSTAFSON, WILLIAM CLAY · 2012 to 2014
$552k
Howard Hughes Medical InstituteNCI NIH HHS T32 CA128583NCI NIH HHS U54 CA163155NCI NIH HHS U54CA163155NIGMS NIH HHS NIH GM030637NIGMS NIH HHS R01 GM030637NINDS NIH HHS K08 NS079485NINDS NIH HHS K08NS079485NINDS NIH HHS R00 NS073735
6 · The paper itself

Abstract

Infrared fluorescent proteins (IFPs) are ideal for in vivo imaging, and monomeric versions of these proteins can be advantageous as protein tags or for sensor development. In contrast to GFP, which requires only molecular oxygen for chromophore maturation, phytochrome-derived IFPs incorporate biliverdin (BV) as the chromophore. However, BV varies in concentration in different cells and organisms. Here we engineered cells to express the haeme oxygenase responsible for BV biosynthesis and a brighter monomeric IFP mutant (IFP2.0). Together, these tools improve the imaging capabilities of IFP2.0 compared with monomeric IFP1.4 and dimeric iRFP. By targeting IFP2.0 to the plasma membrane, we demonstrate robust labelling of neuronal processes in Drosophila larvae. We also show that this strategy improves the sensitivity when imaging brain tumours in whole mice. Our work shows promise in the application of IFPs for protein labelling and in vivo imaging.

Indexed as

AnimalsBiliverdineBrain NeoplasmsCrystallography, X-RayDrosophilaFluorescent DyesHEK293 CellsHeme Oxygenase (Decyclizing)HumansInfrared RaysLarvaLuminescent ProteinsMiceMicroscopy, ConfocalNeuroimagingNeuronsBiliverdineFluorescent DyesHeme Oxygenase (Decyclizing)Luminescent ProteinsPhytochrome

Identifiers

PMID24832154
PMCPMC4077998
OpenAlexW2001589031

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.