Evidence map›Paper›PMID 24832090›Full record

ArticlePloS one2014

Probiotics (VSL#3) prevent endothelial dysfunction in rats with portal hypertension: role of the angiotensin system.

Sherzad K Rashid, Noureddine Idris-Khodja, Noureddine Idris Khodja, Cyril Auger, Mahmoud Alhosin, Nelly Boehm, Monique Oswald-Mammosser, Valérie B Schini-Kerth

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 1 pooled it
2.6field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Trial
  4. Review
  5. Review
  6. Article
  7. Review
  8. The Role of Gut Microbiota and Trimethylamine N-oxide in Cardiovascular Diseases.Journal of cardiovascular translational research · 2023
    Review
  9. Review
  10. Probiotics: functional food ingredients with the potential to reduce hypertension.Frontiers in cellular and infection microbiology · 2023
    Review
  11. Article
  12. Review
  13. Review
  14. Article
  15. Review
  16. Article
  17. Probiotics in hepatology: An update.World journal of hepatology · 2021
    Review
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

  • Erratum issued
    Khodja, Noureddine Idris [corrected to Idris-Khodja, Noureddine]
5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Sherzad K RashidUMR CNRS 7213, Laboratoire de Biophotonique et de Pharmacologie, Faculty of Pharmacy, University of Strasbourg, Illkirch, France.
Noureddine Idris-Khodja
Noureddine Idris KhodjaUMR CNRS 7213, Laboratoire de Biophotonique et de Pharmacologie, Faculty of Pharmacy, University of Strasbourg, Illkirch, France.
Cyril AugerUMR CNRS 7213, Laboratoire de Biophotonique et de Pharmacologie, Faculty of Pharmacy, University of Strasbourg, Illkirch, France.
Mahmoud AlhosinUMR CNRS 7213, Laboratoire de Biophotonique et de Pharmacologie, Faculty of Pharmacy, University of Strasbourg, Illkirch, France.
Nelly BoehmInstitut d'Histologie et INSERM U1119, Fédération de Médecine Translationnelle de Strasbourg, Faculty of Medicine, University of Strasbourg, Strasbourg, France.
Monique Oswald-MammosserService de Physiologie et d'Explorations Fonctionnelles, Pôle de Pathologie Thoracique, Hôpitaux Universitaires de Strasbourg, Faculty of Medicine, University of Strasbourg, Strasbourg, France.
Valérie B Schini-KerthUMR CNRS 7213, Laboratoire de Biophotonique et de Pharmacologie, Faculty of Pharmacy, University of Strasbourg, Illkirch, France.
Centre National de la Recherche Scientifique · FRLaboratoire de Biophotonique et Pharmacologie · FRHôpitaux Universitaires de Strasbourg · FRInserm · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimsPortal hypertension characterized by generalized vasodilatation with endothelial dysfunction affecting nitric oxide (NO) and endothelium-dependent hyperpolarization (EDH) has been suggested to involve bacterial translocation and/or the angiotensin system. The possibility that ingestion of probiotics prevents endothelial dysfunction in rats following common bile duct ligation (CBDL) was evaluated.

methodsRats received either control drinking water or the probiotic VSL#3 solution (50 billion bacteria.kg body wt⁻¹.day⁻¹) for 7 weeks. After 3 weeks, rats underwent surgery with either resection of the common bile duct or sham surgery. The reactivity of mesenteric artery rings was assessed in organ chambers, expression of proteins by immunofluorescence and Western blot analysis, oxidative stress using dihydroethidium, and plasma pro-inflammatory cytokine levels by flow cytometry.

resultsBoth NO- and EDH-mediated relaxations to acetylcholine were reduced in the CBDL group compared to the sham group, and associated with a reduced expression of Cx37, Cx40, Cx43, IKCa and SKCa and an increased expression of endothelial NO synthase (eNOS). In aortic sections, increased expression of NADPH oxidase subunits, angiotensin converting enzyme, AT1 receptors and angiotensin II, and formation of ROS and peroxynitrite were observed. VSL#3 prevented the deleterious effect of CBDL on EDH-mediated relaxations, vascular expression of connexins, IKCa, SKCa and eNOS, oxidative stress, and the angiotensin system. VSL#3 prevented the CBDL-induced increased plasma TNF-α, IL-1α and MCP-1 levels.

conclusionsThese findings indicate that VSL#3 ingestion prevents endothelial dysfunction in the mesenteric artery of CBDL rats, and this effect is associated with an improved vascular oxidative stress most likely by reducing bacterial translocation and the local angiotensin system.

Indexed as

AngiotensinsAnimalsBacterial TranslocationBody WeightCommon Bile DuctCytokinesEndotheliumHypertension, PortalInflammationLiverLiver CirrhosisMaleMesenteric ArteriesMitochondriaNitric OxideOrgan SizeAngiotensinsCytokinesNitric OxideReactive Oxygen Species

Identifiers

PMID24832090
PMCPMC4022585
OpenAlexW2056666616

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.