ArticlePloS one2014
The analysis of the inflorescence miRNome of the orchid Orchis italica reveals a DEF-like MADS-box gene as a new miRNA target.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Morphological and Transcriptome Analysis of the Near-Threatened OrchidPlants (Basel, Switzerland) · 2025Article
- Small RNA profiling reveals that an ovule-specific microRNA, cja-miR5179, targets a B-class MADS-box gene in Camellia japonica.Annals of botany · 2023Article
- The Integrated mRNA and miRNA Approach Reveals Potential Regulators of Flowering Time inInternational journal of molecular sciences · 2023Article
- MicroRNA172b-5p/trehalose-6-phosphate synthase module stimulates trehalose synthesis and microRNA172b-3p/AP2-like module accelerates flowering in barley upon drought stress.Frontiers in plant science · 2023Article
- OrchidBase 5.0: updates of the orchid genome knowledgebase.BMC plant biology · 2022Article
- Micro-RNA-RegulatedPlants (Basel, Switzerland) · 2022Article
- Review
- Advances and prospects of orchid research and industrialization.Horticulture research · 2022Article
- Analysis of miRNA-mediated regulation of flowering induction in Lilium × formolongi.BMC plant biology · 2021Article
- EgmiR5179 Regulates Lipid Metabolism by Targeting EgMADS16 in the Mesocarp of Oil Palm (Frontiers in plant science · 2021Article
- Article
- Evolutionary Conservation of the Orchid MYB Transcription Factors DIV, RAD, and DRIF.Frontiers in plant science · 2019Article
- The High-Throughput Analyses Era: Are We Ready for the Data Struggle?High-throughput · 2018Article
- Post genomics era for orchid research.Botanical studies · 2017Review
- Transcriptome-Wide Identification and Expression Analysis of DIVARICATA- and RADIALIS-Like Genes of the Mediterranean Orchid Orchis italica.Genome biology and evolution · 2017Article
- Characterization and Function of MicroRNAFrontiers in plant science · 2017Article
- Analysis of the TCP genes expressed in the inflorescence of the orchid Orchis italica.Scientific reports · 2015Article
- Characterization and comparative profiling of the small RNA transcriptomes in two phases of flowering in Cymbidium ensifolium.BMC genomics · 2015Article
- A de novo floral transcriptome reveals clues into Phalaenopsis orchid flower development.PloS one · 2015Article
- De novo transcriptome assembly from inflorescence of Orchis italica: analysis of coding and non-coding transcripts.PloS one · 2014Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant microRNAs (miRNAs) are small, regulatory non-coding RNAs involved in a wide range of biological processes, from organ development to response to stimuli. In recent years, an increasing number of studies on model plant species have highlighted the evolutionary conservation of a high number of miRNA families and the existence of taxon-specific ones. However, few studies have examined miRNAs in non-model species such as orchids, which are characterized by highly diversified floral structures and pollination strategies. Therefore, we analysed a small RNA library of inflorescence tissue of the Mediterranean orchid Orchis italica to increase the knowledge on miRNAs in a non-model plant species. The high-throughput sequencing and analysis of a small RNA library of inflorescence of O. italica revealed 23 conserved and 161 putative novel miRNA families. Among the putative miRNA targets, experimental validation demonstrated that a DEF-like MADS-box transcript is cleaved by the homolog of miR5179 of O. italica. The presence of conserved miRNA families in the inflorescence of O. italica indicates that the basic developmental flower regulatory mechanisms mediated by miRNAs are maintained through evolution. Because, according to the "orchid code" theory, DEF-like genes exert a key function in the diversification of tepals and lip, the cleavage-mediated inhibitory activity of miR5179 on a OitaDEF-like transcript suggests that, in orchids, miRNAs play an important role in the diversification of the perianth organs.
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