ArticleMolecular plant pathology2014
Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis.
Article in Molecular plant pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- The Moniliophthora roreri Effector MrB1 Affects Cellular Homeostasis and Induces Genotype-Dependent Necrosis in Theobroma cacao.Molecular plant pathology · 2026Article
- Identification of resistance sources and genomic regions regulating spot blotch resistance in Asian bread wheat (Triticum aestivum L.) via genome-wide association study.The plant genome · 2026Article
- Discovery of Two Novel Phthalide Phytotoxins from the Wheat Tan Spot Fungal PathogenJournal of agricultural and food chemistry · 2024Article
- Spatiotemporal patterns of wheat response to Pyrenophora tritici-repentis in asymptomatic regions revealed by transcriptomic and X-ray fluorescence microscopy analyses.Journal of experimental botany · 2023Article
- A global pangenome for the wheat fungal pathogenMicrobial genomics · 2022Article
- Variability in an effector gene promoter of a necrotrophic fungal pathogen dictates epistasis and effector-triggered susceptibility in wheat.PLoS pathogens · 2022Article
- Review
- Pathogens pulling the strings: Effectors manipulating salicylic acid and phenylpropanoid biosynthesis in plants.Molecular plant pathology · 2021Review
- "Wax On, Wax Off": In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Genetic characterization of adult-plant resistance to tan spot (syn, yellow spot) in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- An optimized sporulation method for the wheat fungal pathogen Pyrenophora tritici-repentis.Plant methods · 2021Article
- Synchrotron X-ray fluorescence microscopy-enabled elemental mapping illuminates the 'battle for nutrients' between plant and pathogen.Journal of experimental botany · 2021Article
- Transcriptome Analysis and Cell Morphology ofGenes · 2021Article
- The identification and deletion of the polyketide synthase-nonribosomal peptide synthase gene responsible for the production of the phytotoxic triticone A/B in the wheat fungal pathogen Pyrenophora tritici-repentis.Environmental microbiology · 2019Article
- A new PacBio genome sequence of an Australian Pyrenophora tritici-repentis race 1 isolate.BMC research notes · 2019Article
- Heterologous Expression of theFrontiers in microbiology · 2019Article
- Discovering new alleles for yellow spot resistance in the Vavilov wheat collection.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Exploration of wheat and pathogen transcriptomes during tan spot infection.BMC research notes · 2018Article
- Proteomics of the wheat tan spot pathogen Pyrenophora tritici-repentis.BMC research notes · 2018Article
- Comparative genomics of the wheat fungal pathogen Pyrenophora tritici-repentis reveals chromosomal variations and genome plasticity.BMC genomics · 2018Article
Corrections and comments
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The necrotrophic fungal pathogen Pyrenophora tritici-repentis causes tan spot, a major disease of wheat, throughout the world. The proteinaceous effector ToxA is responsible for foliar necrosis on ToxA-sensitive wheat genotypes. The single copy ToxA gene was deleted from a wild-type race 1 P. tritici-repentis isolate via homologous recombination of a knockout construct. Expression of the ToxA transcript was found to be absent in transformants (toxa), as was ToxA protein production in fungal culture filtrates. Plant bioassays were conducted to test transformant pathogenicity. The toxa strains were unable to induce necrosis on ToxA-sensitive wheat genotypes. To our knowledge, this is the first demonstration of a targeted gene knockout in P. tritici-repentis. The ability to undertake gene deletions will facilitate the characterization of other pathogenicity effectors of this economically significant necrotroph.
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