ArticlePLoS computational biology2014
In-vivo real-time control of protein expression from endogenous and synthetic gene networks.
Article in PLoS computational biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed.
- Determining growth rates from bright-field images of budding cells through identifying overlaps.eLife · 2023Article
- L-2L ladder digital-to-analogue converter for dynamics generation of chemical concentrations.Royal Society open science · 2023Article
- Control-Based Continuation: A New Approach to Prototype Synthetic Gene Networks.ACS synthetic biology · 2022Article
- Ratiometric control of cell phenotypes in monostrain microbial consortia.Journal of the Royal Society, Interface · 2022Article
- Cybergenetic control of microbial community composition.Frontiers in bioengineering and biotechnology · 2022Review
- Exploiting Noise, Non-Linearity, and Feedback for Differential Control of Multiple Synthetic Cells with a Single Optogenetic Input.ACS synthetic biology · 2021Article
- Control of mammalian cell-based devices with genetic programming.Current opinion in systems biology · 2021Article
- A light tunable differentiation system for the creation and control of consortia in yeast.Nature communications · 2021Article
- Automatic synchronisation of the cell cycle in budding yeast through closed-loop feedback control.Nature communications · 2021Article
- ChipSeg: An Automatic Tool to Segment Bacterial and Mammalian Cells Cultured in Microfluidic Devices.ACS omega · 2021Article
- A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Toward Engineering Biosystems With Emergent Collective Functions.Frontiers in bioengineering and biotechnology · 2020Article
- A tunable dual-input system for on-demand dynamic gene expression regulation.Nature communications · 2019Article
- Sliding mode controller-observer pair for p53 pathway.IET systems biology · 2019Article
- Quantitative Characterization of α-Synuclein Aggregation in Living Cells through Automated Microfluidics Feedback Control.Cell reports · 2019Article
- Biological signal generators: integrating synthetic biology tools andCurrent opinion in systems biology · 2019Article
- Rational engineering of synthetic microbial systems: from single cells to consortia.Current opinion in microbiology · 2018Review
- System-based strategies for p53 recovery.IET systems biology · 2018Article
- NF-κB, the Importance of Being Dynamic: Role and Insights in Cancer.Biomedicines · 2018Review
- Mathematical Modeling Reveals the Role of Hypoxia in the Promotion of Human Mesenchymal Stem Cell Long-Term Expansion.Stem cells international · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
We describe an innovative experimental and computational approach to control the expression of a protein in a population of yeast cells. We designed a simple control algorithm to automatically regulate the administration of inducer molecules to the cells by comparing the actual protein expression level in the cell population with the desired expression level. We then built an automated platform based on a microfluidic device, a time-lapse microscopy apparatus, and a set of motorized syringes, all controlled by a computer. We tested the platform to force yeast cells to express a desired fixed, or time-varying, amount of a reporter protein over thousands of minutes. The computer automatically switched the type of sugar administered to the cells, its concentration and its duration, according to the control algorithm. Our approach can be used to control expression of any protein, fused to a fluorescent reporter, provided that an external molecule known to (indirectly) affect its promoter activity is available.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.