ArticleInternational journal of molecular sciences2014
Lyophilized platelet-rich fibrin (PRF) promotes craniofacial bone regeneration through Runx2.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.
- Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization.International journal of molecular sciences · 2020Pooled it
- Evaluation of osteoblastic activity in extraction sockets treated with platelet-rich fibrin.Medicina oral, patologia oral y cirugia bucal · 2015Trial
- Full-Thickness Regeneration of the Hard Palate Using Bioengineered Mucoperiosteal Scaffolds in a Porcine Model.Biomimetics (Basel, Switzerland) · 2026Article
- Bio-inks with PRF Increase Human Osteosarcoma Cell Line (SaOS-2) Viability in Extrusion-Based 3D-Bioprinted Constructs.Annals of biomedical engineering · 2026Article
- A novel vascularized hydrogel by encapsulation of lyophilized platelet-rich fibrin into gelatin methacryloyl hydrogel for bone regeneration.Journal of materials science. Materials in medicine · 2025Article
- Regenerative effect of lyophilized dental follicle mesenchymal stem cells and platelet-rich fibrin in skin wounds in geriatric and young rats.Scientific reports · 2025Article
- Advancements in Lyophilized platelet-rich fibrin for tissue regeneration.Archives of dermatological research · 2025Article
- Mesenchymal stem cells in PRP and PRF containing poly(3-caprolactone)/gelatin Scaffold: a comparative in-vitro study.Cell and tissue banking · 2024Article
- Review
- Plasma Rich in Growth Factors in Bone Regeneration: The Proximity to the Clot as a Differential Factor in Osteoblast Cell Behaviour.Dentistry journal · 2024Article
- The Effects of Platelet-Rich Fibrin in the Behavior of Mineralizing Cells Related to Bone Tissue Regeneration-A Scoping Review of In Vitro Evidence.Journal of functional biomaterials · 2023Article
- The Effect of Leukocyte- and Platelet-Rich Fibrin on Central and Peripheral Nervous System Neurons-Implications for Biomaterial Applicability.International journal of molecular sciences · 2023Article
- Standardization of Animal Models and Techniques for Platelet-Rich Fibrin Production: A Narrative Review and Guideline.Bioengineering (Basel, Switzerland) · 2023Review
- Evolution and Clinical Advances of Platelet-Rich Fibrin in Musculoskeletal Regeneration.Bioengineering (Basel, Switzerland) · 2023Review
- In Vitro Assessment of Lyophilized Advanced Platelet-Rich Fibrin from Dogs in Promotion of Growth Factor Release and Wound Healing.Veterinary sciences · 2022Article
- Review
- Blood Clots versus PRF: Activating TGF-β Signaling and Inhibiting Inflammation In Vitro.International journal of molecular sciences · 2022Article
- Encapsulation of lyophilized platelet-rich fibrin in alginate-hyaluronic acid hydrogel as a novel vascularized substitution for myocardial infarction.Bioactive materials · 2022Article
- Editorial: Established and Novel Roles of Platelets in Health and Disease.Frontiers in cardiovascular medicine · 2022Article
- Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulatingAnnals of translational medicine · 2022Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Freeze-drying is an effective means to control scaffold pore size and preserve its composition. The purpose of the present study was to determine the applicability of lyophilized Platelet-rich fibrin (LPRF) as a scaffold for craniofacial tissue regeneration and to compare its biological effects with commonly used fresh Platelet-rich fibrin (PRF). LPRF caused a 4.8-fold±0.4-fold elevation in Runt-related transcription factor 2 (Runx2) expression in alveolar bone cells, compared to a 3.6-fold±0.2-fold increase when using fresh PRF, and a more than 10-fold rise of alkaline phosphatase levels and mineralization markers. LPRF-induced Runx2 expression only occurred in alveolar bone and not in periodontal or dental follicle cells. LPRF also caused a 1.6-fold increase in osteoblast proliferation (p<0.001) when compared to fresh PRF. When applied in a rat craniofacial defect model for six weeks, LPRF resulted in 97% bony coverage of the defect, compared to 84% for fresh PRF, 64% for fibrin, and 16% without scaffold. Moreover, LPRF thickened the trabecular diameter by 25% when compared to fresh PRF and fibrin, and only LPRF and fresh PRF resulted in the formation of interconnected trabeculae across the defect. Together, these studies support the application of lyophilized PRF as a biomimetic scaffold for craniofacial bone regeneration and mineralized tissue engineering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.