Evidence map›Paper›PMID 24830435›Full record

ArticleGene therapy2014

Short noncoding DNA fragments improve the immune potency of electroporation-mediated HBV DNA vaccination.

J Peng, S Shi, Z Yang, Q Ding, W Hai, H Tang, Y Yang, J R Bernstein, P Peyda, Y Xu

Abstract read
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In one paragraph

Article in Gene therapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

J PengSchool of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
S ShiSchool of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
Z YangSchool of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
Q DingSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
W HaiSchool of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
H TangSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
Y YangSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
J R BernsteinDepartment of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, CA, USA.
P PeydaDepartment of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, CA, USA.
Y Xu1] School of Biomedical Engineering/Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China [2] School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Electroporation (EP)-mediated DNA immunization can elicit effective immune responses in a variety of animals, and is widely used in research studies and clinical trials. However, high-pulse voltage, high DNA dose and multiple immunizations are still required to achieve considerable immune responses. To further improve the efficiency of EP-mediated DNA immunization, many parameters have been tried and optimized in recent years. In our early research, we found that the short noncoding DNA fragments (sf-DNA) can significantly enhance EP-mediated transgene expression of reporter genes. In this study, we tested the effect of sf-DNA on the immune potency of EP-mediated hepatitis B virus (HBV) DNA vaccination in a mouse model. The results show that the use of sf-DNA in EP-mediated HBV DNA vaccination leads to an enhanced expression of the HBV surface antigen, resulting in higher cellular and humoral responses. Furthermore, the immune responses in the sf-DNA-mediated 120 V cm(-1) EP immunization group were higher than that of the 200 V cm(-1) EP without sf-DNA groups. These data suggest that the sf-DNA can be used as an effective helper molecule to improve the immune response of EP-mediated HBV DNA vaccination, which may make the EP-mediated DNA vaccination more effective and suitable for animal and clinical application.

Indexed as

Alkaline PhosphataseAnimalsDisease Models, AnimalElectroporationFemaleGenetic VectorsHepatitis BHepatitis B Surface AntigensHepatitis B VaccinesMiceMice, Inbred ICRMuscle, SkeletalPlasmidsVaccinationVaccines, DNAAlkaline PhosphataseHepatitis B Surface AntigensHepatitis B VaccinesVaccines, DNA

Identifiers

PMID24830435

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.