ArticlePLoS genetics2014
Phosphorylation of a WRKY transcription factor by MAPKs is required for pollen development and function in Arabidopsis.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
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Who cites it
101 citing papers in PubMed, 198 citations in OpenAlex.
- Phosphorylation of the transcription factor TgWRKY47 by TgSnRK1 modulates cone enlargement in the gymnosperm Torreya grandis.The New phytologist · 2026Article
- Genetic structure analysis of Glycyrrhiza species and identification of SNP-loci associated with secondary metabolites in G. glabra.BMC genomics · 2026Article
- The IKU2/RLK7-MAPK-MINI3 signaling module controls endosperm cellularization timing to determine seed size in Arabidopsis.Journal of integrative plant biology · 2026Article
- The OsWRKY15-OsSUT3 Regulatory Module Enhances Rice Pollen Fertility by Mediating Sucrose Allocation to Anthers.Plant biotechnology journal · 2026Article
- The RhMPK3-RhLOB41-RhWRKY9 module orchestrates ethylene-induced petal abscission via dual control of ROS homeostasis in rose.Science advances · 2026Article
- LsMAPK6 Phosphorylates the LsCO Protein to Enhance Its Stability and Transcriptional Activity, Promoting Floral Transition Upon High Temperatures in Lettuce.Plant biotechnology journal · 2026Article
- Beyond osmoprotection: the expanding roles of proline in plant signalling and development.Biologia futura · 2026Review
- MPK3 as a Signalling Hub in Plants: Integrating Plant Growth, Development and Stress Response.Plants (Basel, Switzerland) · 2026Article
- Plant Lipid Droplets and Derived Lipidic Nano-Assemblies: Structure, Biogenesis and Pharmaceutical Applications.Plant biotechnology journal · 2026Review
- MsWRKY49 Modulates Pollen Tube Elongation in Response to Boron Deficiency by Regulating Reactive Oxygen Species Homeostasis in Alfalfa.Plant biotechnology journal · 2026Article
- MdMAPK6-mediated phosphorylation of MdWRKY9 regulates apple fruit ripening through interaction with MdERF5L.Horticulture research · 2025Article
- Phosphorylation of MdWRKY70L by MdMPK6/02G mediates reactive oxygen accumulation to regulate apple fruit senescence.Plant biotechnology journal · 2025Article
- Chemical genetics reveals cross-regulation of plant developmental signaling by the immune peptide-receptor pathway.Science advances · 2025Article
- Comparative gametogenesis and genomic signatures associated with pollen sterility in the seedless mutant of grapevine.BMC plant biology · 2025Article
- Subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39 regulate immune homeostasis and stomatal opening in Arabidopsis thaliana.The New phytologist · 2024Article
- Chemical genetics reveals cross-activation of plant developmental signaling by the immune peptide-receptor pathway.bioRxiv : the preprint server for biology · 2024Article
- Post-Translational Modification of WRKY Transcription Factors.Plants (Basel, Switzerland) · 2024Review
- MAPK20-mediated ATG6 phosphorylation is critical for pollen development inHorticulture research · 2024Article
- Transcription Factors and Their Regulatory Roles in the Male Gametophyte Development of Flowering Plants.International journal of molecular sciences · 2024Review
- Specificity models in MAPK cascade signaling.FEBS open bio · 2023Review
41 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant male gametogenesis involves complex and dynamic changes in gene expression. At present, little is known about the transcription factors involved in this process and how their activities are regulated. Here, we show that a pollen-specific transcription factor, WRKY34, and its close homolog, WRKY2, are required for male gametogenesis in Arabidopsis thaliana. When overexpressed using LAT52, a strong pollen-specific promoter, epitope-tagged WRKY34 is temporally phosphorylated by MPK3 and MPK6, two mitogen-activated protein kinases (MAPKs, or MPKs), at early stages in pollen development. During pollen maturation, WRKY34 is dephosphorylated and degraded. Native promoter-driven WRKY34-YFP fusion also follows the same expression pattern at the protein level. WRKY34 functions redundantly with WRKY2 in pollen development, germination, and pollen tube growth. Loss of MPK3/MPK6 phosphorylation sites in WRKY34 compromises the function of WRKY34 in vivo. Epistasis interaction analysis confirmed that MPK6 belongs to the same genetic pathway of WRKY34 and WRKY2. Our study demonstrates the importance of temporal post-translational regulation of WRKY transcription factors in the control of developmental phase transitions in plants.
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