ArticleDifferentiation; research in biological diversity
Branching morphogenesis of immortalized human bronchial epithelial cells in three-dimensional culture.
Article in Differentiation; research in biological diversity. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Formulation and Characterisation of an Edible Functional Elderflower Extract Gel for Upper Respiratory Tract Soothing.Gels (Basel, Switzerland) · 2026Article
- Hydrogel-Embedded Precision-Cut Lung Slices Model Lung Cancer Premalignancy Ex Vivo.Advanced healthcare materials · 2024Article
- Alveolar Type 2 Epithelial Cell Organoids: Focus on Culture Methods.Biomedicines · 2023Review
- Regenerative medicine: current research and perspective in pediatric surgery.Pediatric surgery international · 2023Review
- Multilineage murine stem cells generate complex organoids to model distal lung development and disease.The EMBO journal · 2020Article
- Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells.Cell stem cell · 2020Article
- 3D culture models for studying branching morphogenesis in the mammary gland and mammalian lung.Biomaterials · 2019Review
- Endothelial YAP1 in Regenerative Lung Growth through the Angiopoietin-Tie2 Pathway.American journal of respiratory cell and molecular biology · 2019Article
- Through the Looking Glass:Applied in vitro toxicology · 2018Review
- Reconstituting Mouse Lungs with Conditionally Reprogrammed Human Bronchial Epithelial Cells.Tissue engineering. Part A · 2018Article
- Identification of a Human Airway Epithelial Cell Subpopulation with Altered Biophysical, Molecular, and Metastatic Properties.Cancer prevention research (Philadelphia, Pa.) · 2017Article
- NF-κB Mediates Mesenchymal Transition, Remodeling, and Pulmonary Fibrosis in Response to Chronic Inflammation by Viral RNA Patterns.American journal of respiratory cell and molecular biology · 2017Article
- Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling.Stem cells translational medicine · 2017Article
- BRD4 mediates NF-κB-dependent epithelial-mesenchymal transition and pulmonary fibrosis via transcriptional elongation.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Article
- Organotypic culture in three dimensions prevents radiation-induced transformation in human lung epithelial cells.Scientific reports · 2016Article
- Regeneration of the lung: Lung stem cells and the development of lung mimicking devices.Respiratory research · 2016Review
- ESE-1 in Early Development: Approaches for the Future.Frontiers in cell and developmental biology · 2016Article
- Acceleration of Lung Regeneration by Platelet-Rich Plasma Extract through the Low-Density Lipoprotein Receptor-Related Protein 5-Tie2 Pathway.American journal of respiratory cell and molecular biology · 2016Article
- Developing human upper, lower, and deep lung airway models: Combining different scaffolds and developing complex co-cultures.Journal of tissue engineeringArticle
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Authors and funding
7 authors.
Funding
Abstract
While mouse models have contributed in our understanding of lung development, repair and regeneration, inherent differences between the murine and human airways requires the development of new models using human airway epithelial cells. In this study, we describe a three-dimensional model system using human bronchial epithelial cells (HBECs) cultured on reconstituted basement membrane. HBECs form complex budding and branching structures on reconstituted basement membrane when co-cultured with human lung fetal fibroblasts. These structures are reminiscent of the branching epithelia during lung development. The HBECs also retain markers indicative of epithelial cell types from both the central and distal airways suggesting their multipotent potential. In addition, we illustrate how the model can be utilized to understand respiratory diseases such as lung cancer. The 3D novel cell culture system recapitulates stromal-epithelial interactions in vitro that can be utilized to understand important aspects of lung development and diseases.
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