ArticlePLoS genetics2014
Spermatid cyst polarization in Drosophila depends upon apkc and the CPEB family translational regulator orb2.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Orb2 RNA-binding activity promotes neural stem cell development and brain growth in Drosophila larvae.Cell reports · 2026Article
- Article
- The C-terminal ZZ domain of the Drosophila ORB2 RNA-binding protein is required for spermatid individualization.G3 (Bethesda, Md.) · 2026Article
- Article
- The Physiological and Pathological Mechanisms of LIN2, LIN7, LIN10 and Their Tripartite Complex.Journal of cellular and molecular medicine · 2025Review
- The CPEB ortholog Orb2 regulates brain size through the TRIM-NHL RNA-binding protein, Brain tumor.bioRxiv : the preprint server for biology · 2025Article
- Orb2 enables rare-codon-enriched mRNA expression during Drosophila neuron differentiation.Nature communications · 2024Article
- Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq ofeLife · 2023Article
- Long-Term Memory Formation inCells · 2023Article
- Article
- Border cell polarity and collective migration require the spliceosome component Cactin.The Journal of cell biology · 2022Article
- Characterization of reproductive proteins in the Mexican fruit fly points towards the evolution of novel functions.Proceedings. Biological sciences · 2022Article
- The 3'UTR of the Drosophila CPEB translation factor gene orb2 plays a crucial role in spermatogenesis.Development (Cambridge, England) · 2021Article
- Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.Nature communications · 2021Article
- The CPEB translational regulator, Orb, functions together with Par proteins to polarize the Drosophila oocyte.PLoS genetics · 2019Article
- Cell polarity and planar cell polarity (PCP) in spermatogenesis.Seminars in cell & developmental biology · 2018Review
- Vangl2 regulates spermatid planar cell polarity through microtubule (MT)-based cytoskeleton in the rat testis.Cell death & disease · 2018Article
- Review
- Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.PLoS genetics · 2017Article
- Establishing and maintaining cell polarity with mRNA localization in Drosophila.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Mature Drosophila sperm are highly polarized cells--on one side is a nearly 2 mm long flagellar tail that comprises most of the cell, while on the other is the sperm head, which carries the gamete's genetic information. The polarization of the sperm cells commences after meiosis is complete and the 64-cell spermatid cyst begins the process of differentiation. The spermatid nuclei cluster to one side of the cyst, while the flagellar axonemes grows from the other. The elongating spermatid bundles are also polarized with respect to the main axis of the testis; the sperm heads are always oriented basally, while the growing tails extend apically. This orientation within the testes is important for transferring the mature sperm into the seminal vesicles. We show here that orienting cyst polarization with respect to the main axis of the testis depends upon atypical Protein Kinase C (aPKC), a factor implicated in polarity decisions in many different biological contexts. When apkc activity is compromised in the male germline, the direction of cyst polarization within this organ is randomized. Significantly, the mechanisms used to spatially restrict apkc activity to the apical side of the spermatid cyst are different from the canonical cross-regulatory interactions between this kinase and other cell polarity proteins that normally orchestrate polarization. We show that the asymmetric accumulation of aPKC protein in the cyst depends on an mRNA localization pathway that is regulated by the Drosophila CPEB protein Orb2. orb2 is required to properly localize and activate the translation of apkc mRNAs in polarizing spermatid cysts. We also show that orb2 functions not only in orienting cyst polarization with respect to the apical-basal axis of the testis, but also in the process of polarization itself. One of the orb2 targets in this process is its own mRNA. Moreover, the proper execution of this orb2 autoregulatory pathway depends upon apkc.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.