ArticleBiocatalysis and biotransformation2013
Improved soluble expression of the gene encoding amylolytic enzyme Amo45 by fusion with the mobile-loop-region of co-chaperonin GroES in
Article in Biocatalysis and biotransformation, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 8 citations in OpenAlex.
- Influence of the mRNA initial region on protein production: a case study using recombinant detoxified pneumolysin as a model.Frontiers in bioengineering and biotechnology · 2023Article
- β-Galactosidase fromInternational journal of molecular sciences · 2019Article
- Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System.Applied and environmental microbiology · 2016Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene encoding the amylolytic enzyme Amo45, originating from a metagenomic project, was retrieved by a consensus primer-based approach for glycoside hydrolase (GH) family 57 enzymes. Family 57 contains mainly uncharacterized proteins similar to archaeal thermoactive amylopullulanases. For characterization of these family members soluble, active enzymes have to be produced in sufficient amounts. Heterologous expression of
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Registered trials
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