ArticleNucleic acids research2014
Error removal in microchip-synthesized DNA using immobilized MutS.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Engineered eMutS based magnetic separation system for high-fidelity DNA synthesis.Synthetic and systems biotechnology · 2026Article
- The Influence of the Capping Step During Solid-Phase Phosphoramidite Synthesis of Oligonucleotides on Synthetic Errors in Oligonucleotides.Molecules (Basel, Switzerland) · 2025Article
- Long oligos: direct chemical synthesis of genes with up to 1728 nucleotides.Chemical science · 2025Article
- POSoligo software for in vitro gene synthesis.Scientific reports · 2024Article
- Serial enrichment of heteroduplex DNA using a MutS-magnetic bead system.Biotechnology journal · 2023Article
- Oligo Pools as an Affordable Source of Synthetic DNA for Cost-Effective Library Construction in Protein- and Metabolic Pathway Engineering.Chembiochem : a European journal of chemical biology · 2022Review
- Purification of multiplex oligonucleotide libraries by synthesis and selection.Nature biotechnology · 2022Article
- Review
- Synonymous genome recoding: a tool to explore microbial biology and new therapeutic strategies.Nucleic acids research · 2019Review
- A systematic comparison of error correction enzymes by next-generation sequencing.Nucleic acids research · 2017Article
- High-fidelity de novo synthesis of pathways using microchip-synthesized oligonucleotides and general molecular biology equipment.Scientific reports · 2017Article
- High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery.Microbial cell factories · 2017Article
- Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology.Cold Spring Harbor perspectives in biology · 2017Review
- Next generation gene synthesis: From microarrays to genomes.Engineering in life sciences · 2017Review
- Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins.BMC biotechnology · 2016Article
- Platform for high-throughput antibody selection using synthetically-designed antibody libraries.New biotechnology · 2016Article
- Multiplex pairwise assembly of array-derived DNA oligonucleotides.Nucleic acids research · 2016Article
- Effective Optimization of Antibody Affinity by Phage Display Integrated with High-Throughput DNA Synthesis and Sequencing Technologies.PloS one · 2015Article
- A method for multiplex gene synthesis employing error correction based on expression.PloS one · 2015Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of economical de novo gene synthesis methods using microchip-synthesized oligonucleotides has been limited by their high error rates. In this study, a low-cost, effective and improved-throughput (up to 32 oligos per run) error-removal method using an immobilized cellulose column containing the mismatch binding protein MutS was produced to generate high-quality DNA from oligos, particularly microchip-synthesized oligonucleotides. Error-containing DNA in the initial material was specifically retained on the MutS-immobilized cellulose column (MICC), and error-depleted DNA in the eluate was collected for downstream gene assembly. Significantly, this method improved a population of synthetic enhanced green fluorescent protein (720 bp) clones from 0.93% to 83.22%, corresponding to a decrease in the error frequency of synthetic gene from 11.44/kb to 0.46/kb. In addition, a parallel multiplex MICC error-removal strategy was also evaluated in assembling 11 genes encoding ∼21 kb of DNA from 893 oligos. The error frequency was reduced by 21.59-fold (from 14.25/kb to 0.66/kb), resulting in a 24.48-fold increase in the percentage of error-free assembled fragments (from 3.23% to 79.07%). Furthermore, the standard MICC error-removal process could be completed within 1.5 h at a cost as low as $0.374 per MICC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.