ArticleClinical infectious diseases : an official publication of the Infectious Diseases Society of America2014
Detection of Rickettsia rickettsii, Rickettsia parkeri, and Rickettsia akari in skin biopsy specimens using a multiplex real-time polymerase chain reaction assay.
Article in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 72 citations in OpenAlex.
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- Newly Recognized Spotted Fever Group Rickettsia as Cause of Severe Rocky Mountain Spotted Fever-Like Illness, Northern California, USA.Emerging infectious diseases · 2024Article
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- A Case of Critical Japanese Spotted Fever in Zhejiang, China.Infection and drug resistance · 2023Article
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- A forty-year review of Rocky Mountain spotted fever cases in California shows clinical and epidemiologic changes.PLoS neglected tropical diseases · 2022Review
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- Rickettsial Agents Detected in Ixodid Ticks (Acari: Ixodidae) Collected fromJournal of entomological science · 2022Article
- Spotted fever diagnosis: Experience from a South Indian center.Pathogens and global health · 2021Article
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- New Real-Time PCRs to DifferentiateMolecules (Basel, Switzerland) · 2020Article
- New Jersey-Wide Survey of Spotted Fever GroupThe American journal of tropical medicine and hygiene · 2020Article
- An apparent, locally acquired case of rickettsialpox (Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada · 2020Article
- Diagnosis of spotted fever groupEpidemiology and infection · 2019Review
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4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundRickettsia rickettsii, Rickettsia parkeri, and Rickettsia akari are the most common causes of spotted fever group rickettsioses indigenous to the United States. Infected patients characteristically present with a maculopapular rash, often accompanied by an inoculation eschar. Skin biopsy specimens are often obtained from these lesions for diagnostic evaluation. However, a species-specific diagnosis is achieved infrequently from pathologic specimens because immunohistochemical stains do not differentiate among the causative agents of spotted fever group rickettsiae, and existing polymerase chain reaction (PCR) assays generally target large gene segments that may be difficult or impossible to obtain from formalin-fixed tissues.
methodsThis work describes the development and evaluation of a multiplex real-time PCR assay for the detection of these 3 Rickettsia species from formalin-fixed, paraffin-embedded (FFPE) skin biopsy specimens.
resultsThe multiplex PCR assay was specific at discriminating each species from FFPE controls of unrelated bacterial, viral, protozoan, and fungal pathogens that cause skin lesions, as well as other closely related spotted fever group Rickettsia species.
conclusionsThis multiplex real-time PCR demonstrates greater sensitivity than nested PCR assays in FFPE tissues and provides an effective method to specifically identify cases of Rocky Mountain spotted fever, rickettsialpox, and R. parkeri rickettsiosis by using skin biopsy specimens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.