ArticleThe Journal of physiology1989
Argiopine blocks glutamate-activated single-channel currents on crayfish muscle by two mechanisms.
Article in The Journal of physiology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 30 citations in OpenAlex.
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- An analysis of philanthotoxin block for recombinant rat GluR6(Q) glutamate receptor channels.The Journal of physiology · 1998Article
- Block of open channels of recombinant AMPA receptors and native AMPA/kainate receptors by adamantane derivatives.The Journal of physiology · 1997Article
- The action of philanthotoxin-343 and photolabile analogues on locust (Schistocerca gregaria) muscle.Invertebrate neuroscience : IN · 1995Article
- Different types of glutamate receptors in isolated and identified neurones of the mollusc Planorbarius corneus.The Journal of physiology · 1991Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The effect of the spider venom argiopine on L-glutamate-activated membrane channels of crayfish muscle was investigated using the patch-clamp technique. 2. When 10(-2) M-glutamate and 10(-9) M-argiopine were contained in the pipette solution of a cell-attached patch, bursts of openings of excitatory channels appeared after formation of the patch. These bursts ceased abruptly after variable periods of time in the range of 5 min. Higher concentrations of argiopine (up to 10(-6) M) blocked more rapidly, approximately in proportion to concentration. 3. The block of excitatory channels could be partially or completely reversed by hyperpolarizing the membrane by up to -190 mV from the resting potential. The time constant of the recovery of channel opening decreased with increasing hyperpolarization and was 2 ms with -160 mV hyperpolarization. Switching back from the hyperpolarized level to the resting potential, the time constant for the resulting block was about 3 s (10(-7) M-argiopine). Potential-dependent block by argiopine with similar characteristics was also observed in outside-out patches. 4. Up to argiopine concentrations of 10(-7) M the kinetics of channel openings and of bursts measured in pre-block periods or during reversal of the block by hyperpolarization were indistinguishable from controls. 5. When the potential-dependent block observed in the presence of 10(-6) M-argiopine and 10(-2) M-glutamate was reversed by hyperpolarization, additional short closings occurred during bursts. This 'flickering block' did not change burst length appreciably, but an additional open time component (tau = 0.1 ms) appeared and the average open time per burst was reduced. 6. At least two reaction steps seem necessary to model the behaviour of the potential-dependent block. The flickering block may be described as intermittent blocking of the channel which does not interfere with the reactions between glutamate and the channel.
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