ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
The redistribution of Drosophila vesicular monoamine transporter mutants from synaptic vesicles to large dense-core vesicles impairs amine-dependent behaviors.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- An octopamine-glutamate cotransmitting neuron gates aggressive escalation through layered inhibition inScience advances · 2026Article
- A Syd and RUFY dynein adaptor complex mediates axonal circulation of dense core vesicles.The Journal of cell biology · 2026Article
- Evaluation of the Relationship between Vesicular Monoamine Transporter 2 (VMAT2) Inhibition and Neurologic Adverse Events in Approved Drugs.ACS pharmacology & translational science · 2026Article
- Control of Aedes albopictus populations by silencing of the vesicular GABA transporter (vgat) and the vesicular monoamine transporter (vmat) genes using recombinant Chlorella shRNA.Parasites & vectors · 2025Article
- Genetically-encoded markers for confocal visualization of single dense core vesicles.Communications biology · 2025Article
- Genetically-encoded markers for confocal visualization of single dense core vesicles.Research square · 2024Article
- Constitutive and Conditional Epitope Tagging of Endogenous G-Protein-Coupled Receptors inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Neurotoxicology of dopamine: Victim or assailant?Neurotoxicology · 2024Review
- Octopamine in the mushroom body circuitry for learning and memory.Learning & memory (Cold Spring Harbor, N.Y.) · 2024Review
- Precise CRISPR-Cas9-mediated mutation of a membrane trafficking domain in theCurrent research in physiology · 2023Article
- Regulation and modulation of biogenic amine neurotransmission inFrontiers in physiology · 2023Review
- Sleep deprivation results in diverse patterns of synaptic scaling across the Drosophila mushroom bodies.Current biology : CB · 2021Article
- First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory.PloS one · 2021Article
- Vesicular neurotransmitter transporters in Drosophila melanogaster.Biochimica et biophysica acta. Biomembranes · 2020Review
- Deficits in the vesicular acetylcholine transporter alter lifespan and behavior in adult Drosophila melanogaster.Neurochemistry international · 2020Article
- Overexpression of the vesicular acetylcholine transporter disrupts cognitive performance and causes age-dependent locomotion decline in Drosophila.Molecular and cellular neurosciences · 2020Article
- Octopamine neuron dependent aggression requires dVGLUT from dual-transmitting neurons.PLoS genetics · 2020Article
- Article
- Ethanol stimulates the in vivo axonal movement of neuropeptide dense-core vesicles in Drosophila motor neurons.Journal of neurochemistry · 2018Article
- Article
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15 authors.
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Abstract
Monoamine neurotransmitters are stored in both synaptic vesicles (SVs), which are required for release at the synapse, and large dense-core vesicles (LDCVs), which mediate extrasynaptic release. The contributions of each type of vesicular release to specific behaviors are not known. To address this issue, we generated mutations in the C-terminal trafficking domain of the Drosophila vesicular monoamine transporter (DVMAT), which is required for the vesicular storage of monoamines in both SVs and LDCVs. Deletion of the terminal 23 aa (DVMAT-Δ3) reduced the rate of endocytosis and localization of DVMAT to SVs, but supported localization to LDCVs. An alanine substitution mutation in a tyrosine-based motif (DVMAT-Y600A) also reduced sorting to SVs and showed an endocytic deficit specific to aminergic nerve terminals. Redistribution of DVMAT-Y600A from SV to LDCV fractions was also enhanced in aminergic neurons. To determine how these changes might affect behavior, we expressed DVMAT-Δ3 and DVMAT-Y600A in a dVMAT null genetic background that lacks endogenous dVMAT activity. When expressed ubiquitously, DVMAT-Δ3 showed a specific deficit in female fertility, whereas DVMAT-Y600A rescued behavior similarly to DVMAT-wt. In contrast, when expressed more specifically in octopaminergic neurons, both DVMAT-Δ3 and DVMAT-Y600A failed to rescue female fertility, and DVMAT-Y600A showed deficits in larval locomotion. DVMAT-Y600A also showed more severe dominant effects than either DVMAT-wt or DVMAT-Δ3. We propose that these behavioral deficits result from the redistribution of DVMAT from SVs to LDCVs. By extension, our data suggest that the balance of amine release from SVs versus that from LDCVs is critical for the function of some aminergic circuits.
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