ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
Optogenetic manipulation of activity and temporally controlled cell-specific ablation reveal a role for MCH neurons in sleep/wake regulation.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
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Who cites it
117 citing papers in PubMed, 217 citations in OpenAlex.
- Accumulated deep sleep is a powerful predictor of LH pulse onset in pubertal children.The Journal of clinical endocrinology and metabolism · 2015Trial
- Associations between REM sleep EEG slowing and brain cholinergic denervation in aging and Mild Cognitive Impairment.Molecular psychiatry · 2026Article
- The sleep-wake cycle: molecular mechanisms, disorders, and therapeutic targets in the context of energy metabolism.Signal transduction and targeted therapy · 2026Review
- Hypothalamic MCH neurons links tau pathology to sleep disruption.bioRxiv : the preprint server for biology · 2026Article
- Orexinergic Dysregulation in Major Depressive Disorder: Insights from a Prospective Cohort Study Evaluating MADRS, PSQI, and MoCA Scores.Indian journal of psychological medicine · 2026Article
- Wavelength-dependent sleep state manipulation using light pulses in Pogona vitticeps.Communications biology · 2026Article
- Pth4 neurons define a novel hypothalamic circuit that promotes sleep via brainstem monoaminergic neurons.Current biology : CB · 2026Article
- Sleep Disorders in Neurodegenerative Diseases with Dementia: A Comprehensive Review.Journal of clinical medicine · 2025Review
- Pth4 neurons define a novel hypothalamic circuit that promotes sleep via brainstem monoaminergic neurons.bioRxiv : the preprint server for biology · 2025Article
- Neuropeptidergic Input from the Lateral Hypothalamus to the Suprachiasmatic Nucleus Alters the Circadian Period in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- The role of orexin and MCH neurons in the hypothalamus in sleep-wake regulation and learning-forgetting balance.Frontiers in neuroscience · 2025Review
- Association of Changes in Cerebral and Hypothalamic Structure With Sleep Dysfunction in Patients With Genetic Frontotemporal Dementia.Neurology · 2024Observational
- An Electroencephalogram Signature of Melanin-Concentrating Hormone Neuron Activities Predicts Cocaine Seeking.Biological psychiatry · 2024Article
- A hypothalamus-lateral periaqueductal gray GABAergic neural projection facilitates arousal following sevoflurane anesthesia in mice.CNS neuroscience & therapeutics · 2024Article
- Ventral subiculum promotes wakefulness through several pathways in male mice.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2024Article
- An EEG Signature of MCH Neuron Activities Predicts Cocaine Seeking.bioRxiv : the preprint server for biology · 2024Article
- Hypothalamic MCH Neurons: From Feeding to Cognitive Control.Function (Oxford, England) · 2024Review
- Prostaglandin EThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Prefrontal Cortical Regulation of REM Sleep.Research square · 2023Article
- Prefrontal cortical regulation of REM sleep.Nature neuroscience · 2023Article
57 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 6 institutions in 2 countries.
Funding
Abstract
Melanin-concentrating hormone (MCH) is a neuropeptide produced in neurons sparsely distributed in the lateral hypothalamic area. Recent studies have reported that MCH neurons are active during rapid eye movement (REM) sleep, but their physiological role in the regulation of sleep/wakefulness is not fully understood. To determine the physiological role of MCH neurons, newly developed transgenic mouse strains that enable manipulation of the activity and fate of MCH neurons in vivo were generated using the recently developed knockin-mediated enhanced gene expression by improved tetracycline-controlled gene induction system. The activity of these cells was controlled by optogenetics by expressing channelrhodopsin2 (E123T/T159C) or archaerhodopsin-T in MCH neurons. Acute optogenetic activation of MCH neurons at 10 Hz induced transitions from non-REM (NREM) to REM sleep and increased REM sleep time in conjunction with decreased NREM sleep. Activation of MCH neurons while mice were in NREM sleep induced REM sleep, but activation during wakefulness was ineffective. Acute optogenetic silencing of MCH neurons using archaerhodopsin-T had no effect on any vigilance states. Temporally controlled ablation of MCH neurons by cell-specific expression of diphtheria toxin A increased wakefulness and decreased NREM sleep duration without affecting REM sleep. Together, these results indicate that acute activation of MCH neurons is sufficient, but not necessary, to trigger the transition from NREM to REM sleep and that MCH neurons also play a role in the initiation and maintenance of NREM sleep.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.