ArticleProceedings of the National Academy of Sciences of the United States of America2014
Polarized deposition of basement membrane proteins depends on Phosphatidylinositol synthase and the levels of Phosphatidylinositol 4,5-bisphosphate.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Chondroitin sulfate regulates proliferation of Drosophila intestinal stem cells.PLoS genetics · 2025Article
- Prickle1-driven basement membrane deposition of the iPSC-derived embryoid bodies is separable from the establishment of apicobasal polarity.Cell proliferation · 2024Article
- Matrix Metalloproteinases in the Periodontium-Vital in Tissue Turnover and Unfortunate in Periodontitis.International journal of molecular sciences · 2024Review
- Finishing the egg.Genetics · 2024Article
- Genomic and cytogenetic analysis of the Ceratitis capitata temperature-sensitive lethal region.G3 (Bethesda, Md.) · 2023Article
- Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis.Journal of visualized experiments : JoVE · 2022Article
- Kinesin-directed secretion of basement membrane proteins to a subdomain of the basolateral surface in Drosophila epithelial cells.Current biology : CB · 2022Article
- Crosstalk between basal extracellular matrix adhesion and building of apical architecture during morphogenesis.Biology open · 2021Review
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- Cellular homeostasis in theMolecular biology of the cell · 2020Article
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- Oriented basement membrane fibrils provide a memory for F-actin planar polarization via the Dystrophin-Dystroglycan complex during tissue elongation.Development (Cambridge, England) · 2020Article
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- Cell polarization: From epithelial cells to odontoblasts.European journal of cell biology · 2019Review
- The Absence of Laminin α4 in Male Mice Results in Enhanced Energy Expenditure and Increased Beige Subcutaneous Adipose Tissue.Endocrinology · 2018Article
- Laminins in Epithelial Cell Polarization: Old Questions in Search of New Answers.Cold Spring Harbor perspectives in biology · 2017Review
- Dual role of BMP signaling in the regulation of Drosophila intestinal stem cell self-renewal.Fly · 2017Article
- Stratum, a Homolog of the Human GEF Mss4, Partnered with Rab8, Controls the Basal Restriction of Basement Membrane Proteins in Epithelial Cells.Cell reports · 2017Article
- The Hidden Conundrum of Phosphoinositide Signaling in Cancer.Trends in cancer · 2016Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The basement membrane (BM), a specialized sheet of the extracellular matrix contacting the basal side of epithelial tissues, plays an important role in the control of the polarized structure of epithelial cells. However, little is known about how BM proteins themselves achieve a polarized distribution. Here, we identify phosphatidylinositol 4,5-bisphosphate (PIP2) as a critical regulator of the polarized secretion of BM proteins. A decrease of PIP2 levels, in particular through mutations in Phosphatidylinositol synthase (Pis) and other members of the phosphoinositide pathway, leads to the aberrant accumulation of BM components at the apical side of the cell without primarily affecting the distribution of apical and basolateral polarity proteins. In addition, PIP2 controls the apical and lateral localization of Crag (Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein), a factor specifically required to prevent aberrant apical secretion of BM. We propose that PIP2, through the control of Crag's subcellular localization, restricts the secretion of BM proteins to the basal side.
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