ArticleThe Journal of biological chemistry2014
Expression of the bacterial type III effector DspA/E in Saccharomyces cerevisiae down-regulates the sphingolipid biosynthetic pathway leading to growth arrest.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Structural Insights Into the Role of RxLR Effectors in the Arms Race Between Oomycetes and Plants.Molecular plant pathology · 2025Review
- The conserved AvrE family of bacterial effectors: functions and targets during pathogenesis.Trends in microbiology · 2025Review
- Extracellular niche establishment by plant pathogens.Nature reviews. Microbiology · 2024Review
- Protein phosphatase 2A: a high-value target of virulence factors.Trends in parasitology · 2023Article
- Article
- Bacterial pathogens deliver water/solute-permeable channels as a virulence strategy.bioRxiv : the preprint server for biology · 2023Article
- "B" Regulatory Subunits of PP2A: Their Roles in Plant Development and Stress Reactions.International journal of molecular sciences · 2023Review
- DspA/E-Triggered Non-Host Resistance againstInternational journal of molecular sciences · 2022Article
- Plant susceptible responses: the underestimated side of plant-pathogen interactions.Biological reviews of the Cambridge Philosophical Society · 2022Review
- What the Wild Things Do: Mechanisms of Plant Host Manipulation by Bacterial Type III-Secreted Effector Proteins.Microorganisms · 2021Review
- Article
- Reduced fire blight susceptibility in apple cultivars using a high-efficiency CRISPR/Cas9-FLP/FRT-based gene editing system.Plant biotechnology journal · 2020Article
- The effector AWR5 from the plant pathogen Ralstonia solanacearum is an inhibitor of the TOR signalling pathway.Scientific reports · 2016Article
- Direct and Indirect Targeting of PP2A by Conserved Bacterial Type-III Effector Proteins.PLoS pathogens · 2016Article
- Yeast as a Heterologous Model System to Uncover Type III Effector Function.PLoS pathogens · 2016Review
- The AvrE superfamily: ancestral type III effectors involved in suppression of pathogen-associated molecular pattern-triggered immunity.Molecular plant pathology · 2015Review
- Article
- Virulence Factors of Erwinia amylovora: A Review.International journal of molecular sciences · 2015Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Erwinia amylovora, the bacterium responsible for fire blight, relies on a type III secretion system and a single injected effector, DspA/E, to induce disease in host plants. DspA/E belongs to the widespread AvrE family of type III effectors that suppress plant defense responses and promote bacterial growth following infection. Ectopic expression of DspA/E in plant or in Saccharomyces cerevisiae is toxic, indicating that DspA/E likely targets a cellular process conserved between yeast and plant. To unravel the mode of action of DspA/E, we screened the Euroscarf S. cerevisiae library for mutants resistant to DspA/E-induced growth arrest. The most resistant mutants (Δsur4, Δfen1, Δipt1, Δskn1, Δcsg1, Δcsg2, Δorm1, and Δorm2) were impaired in the sphingolipid biosynthetic pathway. Exogenously supplied sphingolipid precursors such as the long chain bases (LCBs) phytosphingosine and dihydrosphingosine also suppressed the DspA/E-induced yeast growth defect. Expression of DspA/E in yeast down-regulated LCB biosynthesis and induced a rapid decrease in LCB levels, indicating that serine palmitoyltransferase (SPT), the first and rate-limiting enzyme of the sphingolipid biosynthetic pathway, was repressed. SPT down-regulation was mediated by dephosphorylation and activation of Orm proteins that negatively regulate SPT. A Δcdc55 mutation affecting Cdc55-PP2A protein phosphatase activity prevented Orm dephosphorylation and suppressed DspA/E-induced growth arrest.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.