ArticleHelicobacter2014
Analysis of clinical isolates of Helicobacter pylori in Pakistan reveals high degrees of pathogenicity and high frequencies of antibiotic resistance.
Article in Helicobacter, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 3 of them syntheses that pooled it.
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Who cites it
30 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- The prevalence of clarithromycin-resistantPeerJ · 2023Pooled it
- Clinical relevance of the cagA and vacA s1m1 status and antibiotic resistance in Helicobacter pylori: a systematic review and meta-analysis.BMC infectious diseases · 2022Pooled it
- Antibiotic resistance in Pakistan: a systematic review of past decade.BMC infectious diseases · 2021Pooled it
- Genomic determinants of antibiotic resistance for Helicobacter pylori treatment: a retrospective phenotypic and genotypic observational study.The Lancet. Microbe · 2026Observational
- Synergistic potentiation of antibiotics by chamomile phytochemicals against multidrug-resistant Helicobacter pylori.Gut pathogens · 2025Article
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- Prevalence and etiology ofAnnals of medicine and surgery (2012) · 2023Article
- Drug Resistance Patterns of Commonly Used Antibiotics for the Treatment ofTropical medicine and infectious disease · 2023Review
- Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2022Article
- Microbial Proteins in Stomach Biopsies Associated with Gastritis, Ulcer, and Gastric Cancer.Molecules (Basel, Switzerland) · 2022Article
- Study on the value of antibiotic-resistant gene detection inExperimental and therapeutic medicine · 2022Article
- Detection of clarithromycin resistance and 23SrRNA point mutations in clinical isolates ofSaudi journal of biological sciences · 2022Article
- Helicobacter pylori 23S rRNA gene A2142G, A2143G, T2182C, and C2195T mutations associated with clarithromycin resistance detected in Sudanese patients.BMC microbiology · 2021Article
- Characterization of Domain V Mutations in Clinical Isolates ofInfection and drug resistance · 2021Article
- A retrospective study of the antibiotic-resistant phenotypes and genotypes ofAmerican journal of cancer research · 2021Article
- Novel virulence factorWorld journal of gastroenterology · 2020Review
- Article
- Helicobacter pylori Infections in the Bronx, New York: Surveying Antibiotic Susceptibility and Strain Lineage by Whole-Genome Sequencing.Journal of clinical microbiology · 2020Article
- Genotype profiles ofTherapeutic advances in gastroenterology · 2020Article
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7 authors.
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Abstract
backgroundAntibiotic resistance in Helicobacter pylori contributes to failure in eradicating the infection and is most often due to point and missense mutations in a few key genes.
methodsThe antibiotic susceptibility profiles of H. pylori isolates from 46 Pakistani patients were determined by Etest. Resistance and pathogenicity genes were amplified, and sequences were analyzed to determine the presence of mutations.
resultsA high percentage of isolates (73.9%) were resistant to metronidazole (MTZ), with considerable resistance to clarithromycin (CLR; 47.8%) and amoxicillin (AML; 54.3%) also observed. Relatively few isolates were resistant to tetracycline (TET; 4.3%) or to ciprofloxacin (CIP; 13%). However, most isolates (n = 43) exhibited resistance to one or more antibiotics. MTZ-resistant isolates contained missense mutations in oxygen-independent NADPH nitroreductase (RdxA; 8 mutations found) and NADH flavin oxidoreductase (FrxA; 4 mutations found). In the 23S rRNA gene, responsible for CLR resistance, a new point mutation (A2181G) and 4 previously reported mutations were identified. Pathogenicity genes cagA, dupA, and vacA s1a/m1 were detected frequently in isolates which were also found to be resistant to MTZ, CLR, and AML. A high percentage of CagA and VacA seropositivity was also observed in these patients. Phylogenetic analysis of partial sequences showed uniform distribution of the 3' region of cagA throughout the tree.
conclusionsWe have identified H. pylori isolates in Pakistan which harbor pathogenicity genes and worrying antibiotic resistance profiles as a result of having acquired multiple point and missense mutations. H. pylori eradication regimens should therefore be reevaluated in this setting.
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