ArticlePloS one2014
Finding sequences for over 270 orphan enzymes.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- A computational workflow for the expansion of heterologous biosynthetic pathways to natural product derivatives.Nature communications · 2021Article
- Implementation of homology based and non-homology based computational methods for the identification and annotation of orphan enzymes: using Mycobacterium tuberculosis H37Rv as a case study.BMC bioinformatics · 2020Article
- Enzyme annotation for orphan and novel reactions using knowledge of substrate reactive sites.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Global untargeted serum metabolomic analyses nominate metabolic pathways responsive to loss of expression of the orphan metallo β-lactamase, MBLAC1.Molecular omics · 2018Article
- Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia coli.Nature methods · 2017Article
- Biochemistry and physiology within the framework of the extended synthesis of evolutionary biology.Biology direct · 2016Article
- Characterising Complex Enzyme Reaction Data.PloS one · 2016Article
- Mesaconase Activity of Class I Fumarase Contributes to Mesaconate Utilization by Burkholderia xenovorans.Applied and environmental microbiology · 2015Article
- UniProt: a hub for protein information.Nucleic acids research · 2015Article
- Updates in Rhea--a manually curated resource of biochemical reactions.Nucleic acids research · 2015Article
- Profiling the orphan enzymes.Biology direct · 2014Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Despite advances in sequencing technology, there are still significant numbers of well-characterized enzymatic activities for which there are no known associated sequences. These 'orphan enzymes' represent glaring holes in our biological understanding, and it is a top priority to reunite them with their coding sequences. Here we report a methodology for resolving orphan enzymes through a combination of database search and literature review. Using this method we were able to reconnect over 270 orphan enzymes with their corresponding sequence. This success points toward how we can systematically eliminate the remaining orphan enzymes and prevent the introduction of future orphan enzymes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.