ArticleMolecular biology of the cell2014
Every laboratory with a fluorescence microscope should consider counting molecules.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Protocol for assessing protein expression levels and localization during fission yeast cytokinesis.STAR protocols · 2026Article
- The MO25 protein Pmo25 functions in contractile ring stability and Sid2 localization during cytokinesis.iScience · 2026Article
- Roles of the MO25 protein Pmo25 in contractile-ring stability and localization of the NDR kinase Sid2 during cytokinesis.bioRxiv : the preprint server for biology · 2025Article
- A meiotic driver hijacks an epigenetic reader to disrupt mitosis in noncarrier offspring.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches.Journal of extracellular vesicles · 2024Article
- Purification Analysis, Intracellular Tracking, and Colocalization of Extracellular Vesicles Using Atomic Force and 3D Single-Molecule Localization Microscopy.Analytical chemistry · 2023Article
- Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis.Molecular biology of the cell · 2022Article
- Inherited nuclear pore substructures template post-mitotic pore assembly.Developmental cell · 2021Article
- Comparison of High- and Low-LET Radiation-Induced DNA Double-Strand Break Processing in Living Cells.International journal of molecular sciences · 2020Article
- Cytoplasmic Amplification of Transcriptional Noise Generates Substantial Cell-to-Cell Variability.Cell systems · 2018Article
- Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis.Molecular biology of the cell · 2018Article
- Analysis of interphase node proteins in fission yeast by quantitative and superresolution fluorescence microscopy.Molecular biology of the cell · 2017Article
- Molecular Counting with Localization Microscopy: A Bayesian Estimate Based on Fluorophore Statistics.Biophysical journal · 2017Article
- Model-independent counting of molecules in single-molecule localization microscopy.Molecular biology of the cell · 2016Article
- Resident CAPS on dense-core vesicles docks and primes vesicles for fusion.Molecular biology of the cell · 2016Article
- Real-Time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells.Methods in molecular biology (Clifton, N.J.) · 2016Article
- "Marker of Self" CD47 on lentiviral vectors decreases macrophage-mediated clearance and increases delivery to SIRPA-expressing lung carcinoma tumors.Molecular therapy. Methods & clinical development · 2016Article
- A palette of fluorescent proteins optimized for diverse cellular environments.Nature communications · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Protein numbers in cells determine rates of biological processes, influence the architecture of cellular structures, reveal the stoichiometries of protein complexes, guide in vitro biochemical reconstitutions, and provide parameter values for mathematical modeling. The purpose of this essay is to increase awareness of methods for counting protein molecules using fluorescence microscopy and encourage more cell biologists to report these numbers. We address the state of the field in terms of utility and accuracy of the numbers reported and point readers to references for details of specific techniques and applications.
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