ArticleFertility and sterility2014
Trophoblast retrieval and isolation from the cervix (TRIC) for noninvasive prenatal screening at 5 to 20 weeks of gestation.
Article in Fertility and sterility, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06523543 (Ultra-early Identification of Fetal Chromosomal Characteristics From Extravillous-trophoblast Cells), which is not on this map. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Ultra-early Identification of Fetal Chromosomal Characteristics From Extravillous-trophoblast Cells
Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- Is cervical swab an efficient method for developing a new noninvasive prenatal diagnostic test for numerical and structural chromosome anomalies?Turkish journal of medical sciences · 2021Pooled it
- Trophoblast Retrieval from the Cervix (TRIC) Across Early Gestation: A Multi-Method Assessment of Extravillous Trophoblast Recovery.International journal of molecular sciences · 2026Article
- Article
- Human placentation: foundations and implications for reproductive endocrinology and infertility.Systems biology in reproductive medicine · 2025Review
- Common chromosomal aneuploidy testing using non-invasive trophoblast retrieval and isolation from the cervix (TRIC) in pregnant women.Archives of gynecology and obstetrics · 2025Article
- Limitations of Semi-Automated Immunomagnetic Separation of HLA-G-Positive Trophoblasts from Papanicolaou Smears for Prenatal Genetic Diagnostics.Diagnostics (Basel, Switzerland) · 2025Article
- Cervical EVT isolation for non-invasive fetal HLA typing in early pregnancy is limited by purity and maternal cell contamination; a methodological comparison.Frontiers in immunology · 2025Article
- A Novel Paradigm for Non-Invasive Prenatal Genetic Screening: Trophoblast Retrieval and Isolation from the Cervix (TRIC).Diagnostics (Basel, Switzerland) · 2023Review
- Trophoblast retrieval from the cervical canal to predict abnormal pregnancy early in gestation: a pilot study.BMC pregnancy and childbirth · 2023Article
- Non-invasive prenatal diagnosis (NIPD): current and emerging technologies.Extracellular vesicles and circulating nucleic acids · 2023Review
- Tricky TRIC: A replication study using trophoblast retrieval and isolation from the cervix to study genetic birth defects.Prenatal diagnosis · 2022Article
- Non-invasive prenatal diagnosis (NIPD): how analysis of cell-free DNA in maternal plasma has changed prenatal diagnosis for monogenic disorders.Clinical science (London, England : 1979) · 2022Article
- Nanotechnologies in Obstetrics and Cancer during Pregnancy: A Narrative Review.Journal of personalized medicine · 2022Review
- Placental Models for Evaluation of Nanocarriers as Drug Delivery Systems for Pregnancy Associated Disorders.Biomedicines · 2022Review
- Isolation and Enrichment of Circulating Fetal Cells for NIPD: An Overview.Diagnostics (Basel, Switzerland) · 2021Review
- Isolation of HLA-GRSC advances · 2021Article
- Overview and recent developments in cell-based noninvasive prenatal testing.Prenatal diagnosis · 2021Review
- Enrichment of Placental Trophoblast Cells from Clinical Cervical Samples Using Differences in Surface Adhesion on an Inclined Plane.Annals of biomedical engineering · 2021Article
- Considerations for whole exome sequencing unique to prenatal care.Human genetics · 2020Review
- Optimization Protocol of Fixation Method for Trophoblast Retrieval from the Cervix (TRIC): A Preliminary Study.Diagnostics (Basel, Switzerland) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveTo use trophoblast cells accumulating in the endocervical canal at the beginning of pregnancy for noninvasive prenatal testing.
designProspective, double-blinded test for fetal gender.
settingAcademic medical center. PATIENT(S): Fifty-six women with singleton pregnancies at gestational age 5-20 weeks. INTERVENTION(S): Isolation of fetal cells from resident maternal cells in endocervical specimens using anti-human leukocyte antigen G coupled to magnetic nanoparticles; cell phenotyping immunofluorescently with a panel of trophoblast subtype-specific proteins; DNA integrity assessment with terminal dUTP nick-end labeling (TUNEL); and polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) to detect sex chromosomes in individual cells. MAIN OUTCOME MEASURE(S): Trophoblast phenotype, TUNEL index, and percentage male cells. RESULT(S): The women were given a routine Papanicolaou test; fetal genders were verified from medical records. Recovery after immunomagnetic isolation averaged 746±59 cells across gestational age, with 99% expressing chorionic gonadotropin, whereas the depleted cell fraction expressed none. The isolated cells had an extravillous trophoblast phenotype and intact nuclear DNA (>95%). Fetal gender was determined in 20 specimens without error by PCR. The FISH analysis of isolated cells from male specimens validated their fetal origin. CONCLUSION(S): Noninvasive prenatal testing is feasible beginning at a gestational age of 5 weeks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.