Evidence map›Paper›PMID 24825347›Full record

ArticleThe EMBO journal2014

Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer.

Kira T Pate, Chiara Stringari, Stephanie Sprowl-Tanio, Kehui Wang, Tara TeSlaa, Nate P Hoverter, Miriam M McQuade, Chad Garner, Michelle A Digman, Michael A Teitell and 3 more

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The EMBO journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05120284 (Trial of Dichloroacetate), which is not on this map. Cited by 311 papers.

0numbers the graph read from it
0cells of the map it votes in
311citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05120284 phase2active not recruitingnot on this mapstarted 2022, after this paper: background citation

Trial of Dichloroacetate (DCA) in Glioblastoma Multiforme (GBM)

TypeinterventionalSponsorUniversity of FloridaRan2022 to 2026Enrolled40ConditionsGlioblastoma MultiformeArmsDichloroacetate (DCA), Genotype
3 · Its place in the literature

Who cites it

311 citing papers in PubMed.

  1. Trial
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  4. TruncatingbioRxiv : the preprint server for biology · 2026
    Article
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  15. Review
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251 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Kira T PateDepartment of Microbiology and Molecular Genetics, University of California, Irvine, CA, USA.
Chiara StringariLaboratory of Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA, USA.
Stephanie Sprowl-TanioDepartment of Microbiology and Molecular Genetics, University of California, Irvine, CA, USA.
Kehui WangDepartment of Pathology and Laboratory Medicine, University of California, Irvine, CA, USA.
Tara TeSlaaDepartments of Pathology, Pediatrics, and Bioengineering, David Geffen School of Medicine University of California, Los Angeles, CA, USA.
Nate P HoverterDepartment of Microbiology and Molecular Genetics, University of California, Irvine, CA, USA.
Miriam M McQuadeDepartment of Microbiology and Molecular Genetics, University of California, Irvine, CA, USA.
Chad GarnerDepartment of Epidemiology, University of California, Irvine, CA, USA.
Michelle A DigmanLaboratory of Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA, USA.
Michael A TeitellDepartments of Pathology, Pediatrics, and Bioengineering, David Geffen School of Medicine University of California, Los Angeles, CA, USA.
Robert A EdwardsDepartment of Pathology and Laboratory Medicine, University of California, Irvine, CA, USA.
Enrico GrattonLaboratory of Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, CA, USA.
Marian L WatermanDepartment of Microbiology and Molecular Genetics, University of California, Irvine, CA, USA marian.waterman@uci.edu.

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Christine Hui · 1994 to 2026
$57.9M
Uncover Strategies Used By Biological SystemsP50GM076516 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI LANDER, ARTHUR D · 2007 to 2016
$25.1M
UCLA NIGMS T32 Program AssessmentT32GM007185 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 1985 to 2020
$21.7M
YEAST RIBOSOMAL STALK COMPOSITION IN VIVO BY TWO-PHOTON FCS AND PCH ANALYSISP41RR003155 · NCRR · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI GRATTON, ENRICO · 1986 to 2011
$14.9M
TRD4: Phasor analysisP41GM103540 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI GRATTON, ENRICO · 2012 to 2020
$12.5M
Human Embryonic Stem Cell CoreP01GM081621 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZACK, JEROME A. · 2008 to 2012
$9.3M
RNA trafficking in mitochondriaR01GM073981 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Carla M Koehler, MICHAEL A TEITELL · 2006 to 2026
$5.1M
TCL1 ONCOGENE IN B LYMPHOCYTE DEVELOPMENT AND NEOPLASIAR01CA090571 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TEITELL, MICHAEL A · 2002 to 2015
$3.8M
LEF/TCF Expression in Colon CancerR01CA096878 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI WATERMAN, MARIAN L · 2003 to 2012
$2.7M
Wnt Signaling in Colon CancerR01CA108697 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI WATERMAN, MARIAN L · 2005 to 2015
$2.7M
(PQD5) Mass Profiling Melanoma Responses to Improve Therapy Choices and PrognosisR01CA185189 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI REED, JASON C, TEITELL, MICHAEL A · 2014 to 2017
$1.9M
A Fourth Outcome: DNA Damage and the Differentiation of B CellsR01CA156674 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TEITELL, MICHAEL A · 2011 to 2015
$1.5M
NCI NIH HHS CA096878NCI NIH HHS CA108697NCI NIH HHS CA-62203NCI NIH HHS P30 CA062203NCI NIH HHS P30CA062203NCI NIH HHS R01 CA090571NCI NIH HHS R01 CA096878NCI NIH HHS R01 CA108697NCI NIH HHS R01 CA156674NCI NIH HHS R01CA156674NCI NIH HHS R01 CA185189NCI NIH HHS R01CA185189NCI NIH HHS R01CA90571NCRR NIH HHS P41-RR03155NIAAA NIH HHS U01 AA021838NIGMS NIH HHS P01 GM081621NIGMS NIH HHS P01GM081621NIGMS NIH HHS P41 GM103540NIGMS NIH HHS P50 GM076516NIGMS NIH HHS R01 GM073981NIGMS NIH HHS R01GM073981NIGMS NIH HHS T32 GM007185NIGMS NIH HHS T32GM007185
6 · The paper itself

Abstract

Much of the mechanism by which Wnt signaling drives proliferation during oncogenesis is attributed to its regulation of the cell cycle. Here, we show how Wnt/β-catenin signaling directs another hallmark of tumorigenesis, namely Warburg metabolism. Using biochemical assays and fluorescence lifetime imaging microscopy (FLIM) to probe metabolism in vitro and in living tumors, we observe that interference with Wnt signaling in colon cancer cells reduces glycolytic metabolism and results in small, poorly perfused tumors. We identify pyruvate dehydrogenase kinase 1 (PDK1) as an important direct target within a larger gene program for metabolism. PDK1 inhibits pyruvate flux to mitochondrial respiration and a rescue of its expression in Wnt-inhibited cancer cells rescues glycolysis as well as vessel growth in the tumor microenvironment. Thus, we identify an important mechanism by which Wnt-driven Warburg metabolism directs the use of glucose for cancer cell proliferation and links it to vessel delivery of oxygen and nutrients.

Indexed as

GlycolysisTumor MicroenvironmentWnt Signaling PathwayAnimalsCell Line, TumorColonic NeoplasmsGlucoseHumansMiceMitochondriaNeoplasm ProteinsNeovascularization, PathologicOxygen ConsumptionProtein Serine-Threonine KinasesPyruvate Dehydrogenase Acetyl-Transferring KinaseGlucoseNeoplasm ProteinsPDK1 protein, humanPdk1 protein, mouseProtein Serine-Threonine KinasesPyruvate Dehydrogenase Acetyl-Transferring Kinaseangiogenesiscolon cancerfluorescence lifetime imagingmetabolismWnt

Identifiers

PMID24825347
PMCPMC4194089

What OpenQuestion holds

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Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.