ArticleAnnals of botany2014
The cell wall pectic polymer rhamnogalacturonan-II is required for proper pollen tube elongation: implications of a putative sialyltransferase-like protein.
Article in Annals of botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 63 citations in OpenAlex.
- AtGPP2 encodes a 3-deoxy-manno-octulosonate-8-phosphatase required for the synthesis of KDO in rhamnogalacturonan II.The Plant journal : for cell and molecular biology · 2026Article
- Reliable assessment of pollen quality in Chrysanthemum × morifolium: comparative evaluation of viability tests and germination media.BMC plant biology · 2026Article
- Genome-wide ascertainment and initial functional characterization and expression pattern dissection of the GT92 gene family in cotton.BMC genomics · 2025Article
- OsPRK1/2/3-mediated reactive oxygen species signaling is required for pollen tube germination in rice.Journal of integrative plant biology · 2025Article
- Storming the barricades of rhamnogalacturonan-II synthesis and function.The Plant cell · 2025Review
- PbrBGAL6 promotes pollen tube growth by influencing apical pectin level in Pyrus bretschneideri.BMC genomics · 2025Article
- Putative rhamnogalacturonan-II glycosyltransferase identified through callus gene editing which bypasses embryo lethality.Plant physiology · 2024Article
- The QTL and Candidate Genes Regulating the Early Tillering Vigor Traits of Late-Season Rice in Double-Cropping Systems.International journal of molecular sciences · 2024Article
- Wheat Grains as a Sustainable Source of Protein for Health.Nutrients · 2023Article
- The Cycas genome and the early evolution of seed plants.Nature plants · 2022Article
- Effect of boron toxicity on pollen tube cell wall architecture and the relationship of cell wall components ofFrontiers in plant science · 2022Article
- Signaling at Physical Barriers during Pollen-Pistil Interactions.International journal of molecular sciences · 2021Review
- Article
- Exocytosis and endocytosis: coordinating and fine-tuning the polar tip growth domain in pollen tubes.Journal of experimental botany · 2020Review
- Anionic and zwitterionic moieties as widespread glycan modifications in non-vertebrates.Glycoconjugate journal · 2020Review
- A Complex Journey: Cell Wall Remodeling, Interactions, and Integrity During Pollen Tube Growth.Frontiers in plant science · 2020Review
- Efficient genome editing of Brassica campestris based on the CRISPR/Cas9 system.Molecular genetics and genomics : MGG · 2019Article
- Poplar carbohydrate-active enzymes: whole-genome annotation and functional analyses based on RNA expression data.The Plant journal : for cell and molecular biology · 2019Article
- Evolution of Cell Wall Polymers in Tip-Growing Land Plant Gametophytes: Composition, Distribution, Functional Aspects and Their Remodeling.Frontiers in plant science · 2019Review
- Reconstruction of the sialylation pathway in the ancestor of eukaryotes.Scientific reports · 2018Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsRhamnogalacturonan-II (RG-II) is one of the pectin motifs found in the cell wall of all land plants. It contains sugars such as 2-keto-3-deoxy-d-lyxo-heptulosaric acid (Dha) and 2-keto-3-deoxy-d-manno-octulosonic acid (Kdo), and within the wall RG-II is mostly found as a dimer via a borate diester cross-link. To date, little is known regarding the biosynthesis of this motif. Here, after a brief review of our current knowledge on RG-II structure, biosynthesis and function in plants, this study explores the implications of the presence of a Golgi-localized sialyltransferase-like 2 (SIA2) protein that is possibly involved in the transfer of Dha or Kdo in the RG-II of Arabidopsis thaliana pollen tubes, a fast-growing cell type used as a model for the study of cell elongation.
methodsTwo heterozygous mutant lines of arabidopsis (sia2-1+/- and qrt1 × sia2-2+/-) were investigated. sia2-2+/- was in a quartet1 background and the inserted T-DNA contained the reporter gene β-glucuronidase (GUS) under the pollen-specific promoter LAT52. Pollen germination and pollen tube phenotype and growth were analysed both in vitro and in vivo by microscopy. KEY
resultsSelf-pollination of heterozygous lines produced no homozygous plants in the progeny, which may suggest that the mutation could be lethal. Heterozygous mutants displayed a much lower germination rate overall and exhibited a substantial delay in germination (20 h of delay to reach 30 % of pollen grain germination compared with the wild type). In both lines, mutant pollen grains that were able to produce a tube had tubes that were either bursting, abnormal (swollen or dichotomous branching tip) or much shorter compared with wild-type pollen tubes. In vivo, mutant pollen tubes were restricted to the style, whereas the wild-type pollen tubes were detected at the base of the ovary.
conclusionsThis study highlights that the mutation in arabidopsis SIA2 encoding a sialyltransferase-like protein that may transfer Dha or Kdo on the RG-II motif has a dramatic effect on the stability of the pollen tube cell wall.
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