ArticleThe ISME journal2014
Cross-feeding and interkingdom communication in dual-species biofilms of Streptococcus mutans and Candida albicans.
Article in The ISME journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 130 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
130 citing papers in PubMed, 1 synthesis or guideline pooled it, 230 citations in OpenAlex.
- Candida albicans and Early Childhood Caries: A Systematic Review and Meta-Analysis.Caries research · 2018Pooled it
- Convergent methodologies in prosthetic joint infection research: integrating transdisciplinary approaches to understand and prevent biofilm-driven failure of orthopaedic prostheses.Journal of medical microbiology · 2026Review
- Quorum sensing in streptococci and its peptide-mediated modulation.Bioscience reports · 2026Review
- Analysis and engineering of quorum sensing-based communications between bacteria and fungi.mBio · 2026Review
- Dental caries beyond eradication: microbiome centered and equilibrium driven therapeutic strategies.Archives of microbiology · 2026Review
- Formation of Streptococcus mutans Polymicrobial Biofilms in the Presence of Lactobacillus plantarum and Candida albicans.European journal of dentistry · 2026Article
- Artemisinins inhibited the synergistic cariogenic effect betweenFrontiers in cellular and infection microbiology · 2026Article
- Article
- Pulpitis as a microbial disease: single-cell insights into host responses and diagnostic biomarkers for vital pulp therapy.Journal of oral microbiology · 2026Review
- Mechanisms of caries induced by sugars: a narratives review from microbial metabolism to oral ecological imbalance and public health strategies for caries prevention.Frontiers in cellular and infection microbiology · 2026Review
- Host-pathogen interactions in periodontitis: an integrative interkingdom perspective.Frontiers in immunology · 2026Review
- Ecology of the gut microbiota and colonization resistance: mechanisms and therapeutic implications.FEMS microbiology ecology · 2025Review
- Unraveling novel insights into dual-species cariogenic biofilm formation on aged teeth: a comparative analysis on natural vs artificial bioengineered dentin models.Applied and environmental microbiology · 2025Article
- Review
- Distal gut colonization by oral bacteria during intensive chemotherapy: direct evidence from strain-level analysis of paired samples.NPJ biofilms and microbiomes · 2025Article
- Metabolic Influence ofJournal of fungi (Basel, Switzerland) · 2025Article
- Effects of snake fungal disease (ophidiomycosis) on the skin microbiome across two major experimental scales.Conservation biology : the journal of the Society for Conservation Biology · 2025Article
- Branched-chain amino acid metabolism supports Roseobacteraceae positive interactions in marine biofilms.Applied and environmental microbiology · 2025Article
- V-ATPase contributes to the cariogenicity of Candida albicans- Streptococcus mutans biofilm.NPJ biofilms and microbiomes · 2025Article
- Interactions between non-Journal of oral microbiology · 2025Article
70 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polymicrobial biofilms are of large medical importance, but relatively little is known about the role of interspecies interactions for their physiology and virulence. Here, we studied two human pathogens co-occuring in the oral cavity, the opportunistic fungus Candida albicans and the caries-promoting bacterium Streptococcus mutans. Dual-species biofilms reached higher biomass and cell numbers than mono-species biofilms, and the production of extracellular polymeric substances (EPSs) by S. mutans was strongly suppressed, which was confirmed by scanning electron microscopy, gas chromatography-mass spectrometry and transcriptome analysis. To detect interkingdom communication, C. albicans was co-cultivated with a strain of S. mutans carrying a transcriptional fusion between a green fluorescent protein-encoding gene and the promoter for sigX, the alternative sigma factor of S. mutans, which is induced by quorum sensing signals. Strong induction of sigX was observed in dual-species biofilms, but not in single-species biofilms. Conditioned media from mixed biofilms but not from C. albicans or S. mutans cultivated alone activated sigX in the reporter strain. Deletion of comS encoding the synthesis of the sigX-inducing peptide precursor abolished this activity, whereas deletion of comC encoding the competence-stimulating peptide precursor had no effect. Transcriptome analysis of S. mutans confirmed induction of comS, sigX, bacteriocins and the downstream late competence genes, including fratricins, in dual-species biofilms. We show here for the first time the stimulation of the complete quorum sensing system of S. mutans by a species from another kingdom, namely the fungus C. albicans, resulting in fundamentally changed virulence properties of the caries pathogen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.