ArticleNucleus (Austin, Tex.)
Retrotransposon Alu is enriched in the epichromatin of HL-60 cells.
Article in Nucleus (Austin, Tex.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Repair and misrepair of telomeric DNA in dynamic interactions with PML nuclear bodies and lamin B1 in doxorubicin-treated cancer cells.Nucleus (Austin, Tex.) · 2026Article
- Interphase epichromatin: last refuge for the 30-nm chromatin fiber?Chromosoma · 2021Review
- An evolutionary driver of interspersed segmental duplications in primates.Genome biology · 2020Article
- Linker histone epitopes are hidden by in situ higher-order chromatin structure.Epigenetics & chromatin · 2020Article
- Mesoscale Modeling of Nucleosome-Binding Antibody PL2-6: Mono- versus Bivalent Chromatin Complexes.Biophysical journal · 2020Article
- Whole-genome fingerprint of the DNA methylome during chemically induced differentiation of the human AML cell line HL-60/S4.Biology open · 2020Article
- Article
- Epichromatin and chromomeres: a 'fuzzy' perspective.Open biology · 2018Review
- Jörg Langowski: his scientific legacy and the future it promises.BMC biophysics · 2018Article
- Differential staining of peripheral nuclear chromatin with Acridine orange implies an A-form epichromatin conformation of the DNA.Nucleus (Austin, Tex.) · 2018Article
- Defining the epichromatin epitope.Nucleus (Austin, Tex.) · 2017Article
- Nucleosome repositioning during differentiation of a human myeloid leukemia cell line.Nucleus (Austin, Tex.) · 2017Article
- Transcriptomes reflect the phenotypes of undifferentiated, granulocyte and macrophage forms of HL-60/S4 cells.Nucleus (Austin, Tex.) · 2017Article
- Nucleolar aggresomes mediate release of pericentric heterochromatin and nuclear destruction of genotoxically treated cancer cells.Nucleus (Austin, Tex.) · 2017Article
- How retrotransposons shape genome regulation.Current opinion in genetics & development · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epichromatin, the surface of chromatin facing the nuclear envelope in an interphase nucleus, reveals a "rim" staining pattern with specific mouse monoclonal antibodies against histone H2A/H2B/DNA and phosphatidylserine epitopes. Employing a modified ChIP-Seq procedure on undifferentiated and differentiated human leukemic (HL-60/S4) cells,>95% of assembled epichromatin regions overlapped with Alu retrotransposons. They also exhibited enrichment of the AluS subfamily and of Alu oligomers. Furthermore, mapping epichromatin regions to the human chromosomes revealed highly similar localization patterns in the various cell states and with the different antibodies. Comparisons with available epigenetic databases suggested that epichromatin is neither "classical" heterochromatin nor highly expressing genes, implying another function at the surface of interphase chromatin. A modified chromatin immunoprecipitation procedure (xxChIP) was developed because the studied antibodies react generally with mononucleosomes and lysed chromatin. A second fixation is necessary to securely attach the antibodies to the epichromatin epitopes of the intact nucleus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.