ArticlePloS one2014
Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 88 papers.
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88 citing papers in PubMed, 205 citations in OpenAlex.
- Protocol for model-guided alternating high-temperature thermotherapy to eliminate major viruses from diverse sugarcane cultivars.STAR protocols · 2026Article
- Identification of Reference Genes for RT-qPCR Assays inCurrent issues in molecular biology · 2026Article
- Integrated comparative transcriptomics and WGCNA reveal the core transcriptional network and hub genes regulating sucrose accumulation in Saccharum spp.BMC plant biology · 2026Article
- Analysis of Axillary Bud Germination Regulatory Network in Sugarcane Based on Transcriptome and Weighted Gene Co-Expression Network Analysis.Plants (Basel, Switzerland) · 2026Article
- Coordinated regulation of nitrogen transport and assimilation drives high nitrogen use efficiency in sugarcane.Frontiers in plant science · 2026Article
- Transcriptional reprogramming and co-expression network underlying enhanced ammonium uptake in intraspecific hybrids ofFrontiers in plant science · 2026Article
- Evaluation and validation of reference genes for RT-qPCR normalization in different sweet potato tissues.Scientific reports · 2025Article
- Genome-Wide Identification, Evolution, and Expression Patterns of the Fructose-1,6-Bisphosphatase Gene Family inPlants (Basel, Switzerland) · 2025Article
- Genome-wide analysis of the cellulose synthase gene family in autopolyploid sugarcane (Frontiers in plant science · 2025Article
- Identification of the tetraspanin gene family in sugarcane and its response to sugarcane mosaic virus infection.Frontiers in plant science · 2025Article
- Allele-Specific Hormone Dynamics in Highly Transgressive F2 Biomass Segregants in Sugarcane (Plants (Basel, Switzerland) · 2024Article
- Identification of candidate single-nucleotide polymorphisms (SNPs) and genes associated with sugarcane leaf scald disease.Scientific reports · 2024Article
- Identification and characterization of PAL genes involved in the regulation of stem development in Saccharum spontaneum L.BMC genomic data · 2024Article
- Genomic investigation of duplication, functional conservation, and divergence in the LRR-RLK Family of Saccharum.BMC genomics · 2024Article
- Reference genes for Eucalyptus spp. under Beauveria bassiana inoculation and subsequently infestation by the galling wasp Leptocybe invasa.Scientific reports · 2024Article
- Expression of Trichoderma spp. endochitinase gene improves red rot disease resistance in transgenic sugarcane.PloS one · 2024Article
- Identification and characterization of theFrontiers in plant science · 2024Article
- Transcriptome analysis of sugarcane reveals rapid defense response of SES208 to Xanthomonas albilineans in early infection.BMC plant biology · 2023Article
- Ectopic Expression of SugarcaneInternational journal of molecular sciences · 2023Article
- Screening of Candidate Genes Associated with Brown Stripe Resistance in Sugarcane via BSR-seq Analysis.International journal of molecular sciences · 2022Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method for gene expression analysis requires one or several reference gene(s) acting as normalization factor(s). In order to facilitate gene expression studies in sugarcane (Saccharum officinarum), a non-model plant with limited genome information, the stability of 13 candidate reference genes was evaluated. The geNorm, NormFinder and deltaCt methods were used for selecting stably expressed internal controls across different tissues and under various experimental treatments. These results revealed that, among these 13 candidate reference genes, GAPDH, eEF-1a and eIF-4α were the most stable and suitable for use as normalization factors across all various experimental samples. In addition, APRT could be a candidate for examining the relationship between gene copy number and transcript levels in sugarcane tissue samples. According to the results evaluated by geNorm, combining CUL and eEF-1α in hormone treatment experiments; CAC and CUL in abiotic stress tests; GAPDH, eEF-1a and CUL in all treatment samples plus CAC, CUL, APRT and TIPS-41 in cultivar tissues as groups for normalization would lead to more accurate and reliable expression quantification in sugarcane. This is the first systematic validation of reference genes for quantification of transcript expression profiles in sugarcane. This study should provide useful information for selecting reference genes for more accurate quantification of gene expression in sugarcane and other plant species.
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