ArticleBioMed research international2014
Strain-dependent induction of human enterocyte apoptosis by blastocystis disrupts epithelial barrier and ZO-1 organization in a caspase 3- and 9-dependent manner.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- Molecular Detection ofVeterinary sciences · 2026Article
- An In Vitro Cell Model of Intestinal Barrier Function Using a Low-Cost 3D-Printed Transwell Device and Paper-Based Cell Membrane.International journal of molecular sciences · 2025Article
- Trichinella spiralis excretory/secretory proteins mediated larval invasion via inducing gut epithelial apoptosis and barrier disruption.PLoS neglected tropical diseases · 2025Article
- An Update onMicroorganisms · 2024Review
- Blastocystis colonization and associations with population parameters in Thai adults.PLoS neglected tropical diseases · 2024Article
- The influence of plant extracts on viability of ST3 and ST7 subtypes of Blastocystis sp.Gut pathogens · 2024Article
- Extracellular Cysteine Proteases of Key Intestinal Protozoan Pathogens-Factors Linked to Virulence and Pathogenicity.International journal of molecular sciences · 2023Review
- Presence of Blastocystis in gut microbiota is associated with cognitive traits and decreased executive function.The ISME journal · 2022Article
- Modulation of microRNAs and claudin-7 in Caco-2 cell line treated with Blastocystis sp., subtype 3 soluble total antigen.BMC microbiology · 2022Article
- Interactions between Blastocystis subtype ST4 and gut microbiota in vitro.Parasites & vectors · 2022Article
- Exosomes secreted byFrontiers in medicine · 2022Article
- The synthesis of extracellular vesicles by the protistan parasiteFrontiers in cellular and infection microbiology · 2022Article
- The Infection, Coinfection, and Abundance of Intestinal Protozoa Increase the Serum Levels of IFABP2 and TNF-α in Patients With Rheumatoid Arthritis.Frontiers in medicine · 2022Article
- Blastocystis and Clostridioides difficile: Evidence for a Synergistic Role in Colonization Among IBD Patients with Emphasis on Ulcerative Colitis.The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2021Article
- The influence of selected gastrointestinal parasites on apoptosis in intestinal epithelial cells.Biomolecules · 2020Review
- Surface ultrastructure, protein profile and zymography ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2019Article
- Interactions between a pathogenic Blastocystis subtype and gut microbiota: in vitro and in vivo studies.Microbiome · 2019Article
- Membrane Surface Features ofGenes · 2018Article
- Review
- Exacerbation of colon carcinogenesis by Blastocystis sp.PloS one · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blastocystis is an emerging protistan parasite colonizing the human intestine. It is frequently reported to cause general intestinal symptoms of vomiting, diarrhea, and abdominal pain. We recently demonstrated that Blastocystis rearranged cytoskeletal proteins and induced intestinal epithelial barrier compromise. The effect of Blastocystis on enterocyte apoptosis is unknown, and a possible link between microbially induced enterocyte apoptosis and increased epithelial permeability has yet to be determined. The aim of this study is to assess if Blastocystis induces human enterocyte apoptosis and whether this effect influences human intestinal epithelial barrier function. Monolayers of polarized human colonic epithelial cell-line Caco-2 were incubated with Blastocystis subtype 7 and subtype 4. Assays for both early and late markers of apoptosis, phosphatidylserine externalization, and nuclear fragmentation, respectively, showed that Blastocystis ST-7, but not ST-4, significantly increased apoptosis in enterocytes, suggesting that Blastocystis exhibits host specificity and strain-to-strain variation in pathogenicity. ST-7 also activated Caco-2 caspases 3 and 9 but not 8. ST-7 induced changes in epithelial resistance, permeability, and tight junction (ZO-1) localization. Pretreatment of Caco-2 monolayers with a pan-caspase inhibitor z-VAD-fmk significantly inhibited these changes. This suggests a role for enterocyte apoptosis in Blastocystis-mediated epithelial barrier compromise in the human intestine.
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