Evidence map›Paper›PMID 24822117›Full record

ArticleJournal of thoracic disease2014

The value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in identifying clinically relevant bacteria: a comparison with automated microbiology system.

Chunmei Zhou, Bijie Hu, Xuelian Zhang, Shenglei Huang, Yuzhang Shan, Xiangru Ye

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of thoracic disease, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Performance of the matrix-assisted laser desorption ionization time-of-flight mass spectrometry system for rapid identification of streptococci: a review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017
    Pooled it
  2. Article
  3. Article
  4. Performance of MALDI-TOF Mass Spectrometry in the Philippines.Tropical medicine and infectious disease · 2021
    Article
  5. Article
  6. Article
  7. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) for rapid identification of micro-organisms in the routine clinical microbiology laboratory.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017
    Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Chunmei Zhou1 Department of Clinical Microbiology Laboratory, Zhongshan Hospital, Fudan University, Shanghai 20032, China ; 2 State Key laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, China.
Bijie Hu1 Department of Clinical Microbiology Laboratory, Zhongshan Hospital, Fudan University, Shanghai 20032, China ; 2 State Key laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, China.
Xuelian Zhang1 Department of Clinical Microbiology Laboratory, Zhongshan Hospital, Fudan University, Shanghai 20032, China ; 2 State Key laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, China.
Shenglei Huang1 Department of Clinical Microbiology Laboratory, Zhongshan Hospital, Fudan University, Shanghai 20032, China ; 2 State Key laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, China.
Yuzhang Shan1 Department of Clinical Microbiology Laboratory, Zhongshan Hospital, Fudan University, Shanghai 20032, China ; 2 State Key laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, China.
Xiangru Ye1 Department of Clinical Microbiology Laboratory, Zhongshan Hospital, Fudan University, Shanghai 20032, China ; 2 State Key laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMatrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been developed as a new-type soft ionization mass spectrometry in the recent year. Increasing number of clinical microbiological laboratories consider it as an innovate approach for bacterial identification.

methodsA total of 876 clinical strains, comprising 52 species in 27 genus, were obtained from Fudan University Affiliated Zhongshan Hospital. We compared the identification accuracy of the Vitek MS system (bioMerieux, Marcy l'Etoile) to other conventional methods for bacterial identification. 16S rRNA gene sequencing was performed as a reference identification method in cases of discrepant results.

resultsThe Vitek MS system consistently produced accurate results within minutes of loading, while conventional methods required several hours to produce identification results. Among the 876 isolates, the overall performance of Vitek MS was significantly better than the conventional method both for correct species identification (830, 94.7% vs. 746, 85.2%, respectively, P=0.000).

conclusionsCompared to traditional identification methods, MALDI-TOF MS is a rapid, accurate and economical technique to enhance the clinical value of microorganism identification.

Indexed as

bacteriaidentificationMass spectrometry

Identifiers

PMID24822117
PMCPMC4015012

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.